Cloning, expression, and chromosomal localization of the 140-kilodalton subunit of replication factor C from mice and humans
Journal Article
·
· Molecular and Cellular Biology; (United States)
- Cold Spring Harbor Laboratory, NY (United States)
The authors have isolated a full-length mouse cDNA encoding a lysine-rich protein of 1,131 amino acids with a calculated molecular mass of 126 kDa. The protein binds in a sequence-unspecific manner to DNA, is localized exclusively in the nucleus, and contains a putative ATP binding site and a stretch of 80 amino acids with homology to the carboxy terminus of prokaryotic DNA ligases. On the bases of the following facts, the authors conclude that the isolated cDNA encodes the 140-kDa subunit of mouse replication factor C (mRFC140). (i) The sequence around the ATP binding site shows significant homology to three small subunits of human replication factor C. (ii) Polyclonal antibodies raised against the protein encoded by this cDNA cross-react with the 140-kDa subunit of purified human replication factor C (hRFC140) and recognize in mouse cell extracts an authentic protein with an apparent molecular mass of 130 kDa. (iii) Sequence comparison with a human cDNA isolated by using tryptic peptide sequence information from purified hRFC140 revealed 83% identity of the encoded proteins. The mRFC140 gene is ubiquitously expressed, and two mRNAs approximately 5.0 and 4.5 kb long have been detected. The gene was mapped by in situ hybridization to mouse chromosome 5, and its human homolog was mapped to chromosome 4 (p13-p14). 58 refs., 8 figs.
- OSTI ID:
- 7082337
- Journal Information:
- Molecular and Cellular Biology; (United States), Journal Name: Molecular and Cellular Biology; (United States) Vol. 14:3; ISSN 0270-7306; ISSN MCEBD4
- Country of Publication:
- United States
- Language:
- English
Similar Records
Human DNA ligase I cDNA: Cloning and functional expression in Saccharomyces cerevisiae
Molecular cloning and chromosomal localization of the murine homolog of the human helix-loop-helix gene SCL
cDNA sequence, map, and expression of the murine homolog of GTBP, a DNA mismatch repair gene
Journal Article
·
Sat Sep 01 00:00:00 EDT 1990
· Proceedings of the National Academy of Sciences of the United States of America; (United States)
·
OSTI ID:5381681
Molecular cloning and chromosomal localization of the murine homolog of the human helix-loop-helix gene SCL
Journal Article
·
Thu Jan 31 23:00:00 EST 1991
· Proceedings of the National Academy of Sciences of the United States of America; (United States)
·
OSTI ID:6044957
cDNA sequence, map, and expression of the murine homolog of GTBP, a DNA mismatch repair gene
Journal Article
·
Sun Sep 01 00:00:00 EDT 1996
· Genomics
·
OSTI ID:486314