Molecular cloning and chromosomal localization of the murine homolog of the human helix-loop-helix gene SCL

Journal Article · Fri Feb 01 04:00:00 EST 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.3.869· OSTI ID:6044957

Begley, C G; Visvader, J; Green, A R; Metcalf, D; Gough, N M ^[1]; Aplan, P D; Kirsch, I R ^[2]

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1. Royal Melbourne Hospital, Victoria (Australia)
2. National Cancer Inst., Bethesda, MD (United States)

The human SCL gene is a member of the family of genes that encode the helix-loop-helix (HLH) class of DNA-binding proteins. A murine SCL cDNA was isolated from a normal macrophage cDNA library by using HLH-specific oligonucleotides as hybridiazation probes. The coding region is 987 base pairs and encodes a predicted protein of 34 kDa. The nucleotide sequence of the coding region shows 88% identity to the human SCL gene, and the amino acid sequence is 94% identical. The LHL motif and upstream hydrophilic region are entirely conserved in the murine and human proteins. The identity between the mouse and human sequences was less marked in the 5{prime} and 3{prime} untranslated regions. Two murine SCL transcripts that differ in the 3{prime} noncoding region have been detected in fetal liver and various cell lines. Variation was also observed in the 5{prime} untranslated region. Interestingly, immediately downstream of the protein-termination codon, both the human SCL sequence and the murine homolog share an E-box element - the suggested target site for DNA binding of HLH proteins. The murine SCL homolog was mapped to the central part of chromosome 4.

• OSTI ID: 6044957
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:3; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
CHROMOSOMES
CLONING
DNA
DNA HYBRIDIZATION
DNA-CLONING
GENES
GENETIC MAPPING
HETEROCHROMOSOMES
HYBRIDIZATION
MAPPING
MOLECULAR STRUCTURE
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PROTEINS
RECOMBINANT DNA
TRANSCRIPTION
TRANSCRIPTION FACTORS