Identification and characterization of a human cDNA homologous to yeast SK12
Journal Article
·
· Genomics
OSTI ID:232713
- Asan Institute for Life Sciences, Seoul (Korea, Democratic People`s Republic of); and others
A monoclonal antibody, 170A1, which recognizes a nucleolar peptide of molecular weight 90,000, was raised. The protein was conserved among various vertebrates. To characterize the antigen, we screened a human fetal liver expression library using the monoclonal antibody as a probe. Molecular analyses of immunopositive clones suggested the presence of a novel cDNA. It appeared to be a single-copy gene and encoded about 4- and 5-kb mRNAs. The gene appeared to be expressed in every cell tested so far. Its deduced amino acid sequence revealed an overall homology to recently described yeast SK12. The SK12 gene of Saccharomyces cerevisiae encoded a nucleolar protein that is involved in the antiviral system. We report here the partial human cDNA sequence and the localization of the corresponding gene on chromosome 6p21. 20 refs., 7 figs.
- OSTI ID:
- 232713
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 3 Vol. 25; ISSN 0888-7543; ISSN GNMCEP
- Country of Publication:
- United States
- Language:
- English
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