Molecular cloning of cDNA for the carcinoma-associated antigen GA733-2

Journal Article · Tue May 01 04:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.87.9.3542· OSTI ID:5463747

Szala, S; Froehlich, M; Scollon, M; Kasai, Y; Steplewski, Z; Koprowski, H; Linnenbach, A J ^[1]

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1. Wistar Institute of Anatomy and Biology, Philadelphia, PA (USA)

Defined by monoclonal antibody GA733, the GA733-2 antigen is a cell surface 40-kDa glycoprotein associated with human carcinomas of various origins. Molecular clones for the GA733-2 antigen were isolated from a colorectal carcinoma cell line cDNA library using the high-efficiency COS cell expression system. A 1.4-kilobase cDNA species was enriched by immunoselection with monoclonal antibody. The authenticity of individual clones was established by immunologic and sequence criteria. At the amino acid sequence level, GA733-2 was found to be >99% identical to the previously described KSA antigen defined by monoclonal antibody KS1/4. The amino acid sequence derived from the previously described GA733-related gene, GA733-1, was found to be 49% identical to GA733-2. The positions of 12 cysteine residues in the extracellular domains of the two GA733 antigens are conserved, as is the overall distribution of hydrophobic and hydrophilic residues. A 1.45-kilobase transcript of the GA733-2/KSA gene was found to be expressed in cell lines derived from colorectal and pancreatic carcinoma.

• OSTI ID: 5463747
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 87:9; ISSN 0027-8424; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
AMINO ACIDS
ANIMALS
ANTIBODIES
ANTIGENS
CARBOXYLIC ACIDS
CARCINOMAS
CLONING
CYSTEINE
DISEASES
DNA
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
GLYCOPROTEINS
HYBRIDIZATION
MAMMALS
MAN
MESSENGER-RNA
MOLECULAR STRUCTURE
MONOCLONAL ANTIBODIES
NEOPLASMS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PRIMATES
PROTEINS
RECOMBINANT DNA
RNA
STRUCTURAL CHEMICAL ANALYSIS
THIOLS
VERTEBRATES