Isolation of a cDNA for HSF 2: Evidence for two heat shock factor genes in humans

Journal Article · Thu Aug 15 04:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.16.6911· OSTI ID:5822671

Schuetz, T J; Gallo, G J; Sheldon, L; Kingston, R E ^[1]; Tempst, P ^[2]

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1. Massachusetts General Hospital, Boston (United States) Harvard Medical School, Boston, MA (United States)
2. Harvard Medical School, Boston, MA (United States)

The heat shock response is transcriptionally regulated by an evolutionarily conserved protein termed heat shock factor (HSF). The authors report the purification to homogeneity and the partial peptide sequence of HSF from HeLa cells. The peptide sequence was used to isolate a human cDNA with a predicted open reading frame that has homology to the DNA binding domains of both Saccharomyces cerevisiae and Drosophila HSFs. The cDNA directs the synthesis of a protein that binds to the heat shock element with specificity identical to HeLa HSF and stimulates transcription from a heat shock promoter. The expressed protein cross-reacts with anti-HSF antibodies. Surprisingly, however, this cDNA does not encode all of the peptides obtained from purified HeLa HSF. These peptides are encoded by a distinct human cDNA. HSF1. It therefore appears that there is a human heat shock factor gene family and that at least two separate but related HSF proteins regulate the stress response in humans.

• OSTI ID: 5822671
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:16; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

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