Effect of temperature on the radioiodination of human growth hormone
Journal Article
·
· J. Immunoassay; (United States)
Studies have been undertaken to assess the effect of altering the temperature at which human growth hormone is radioiodinated on the incorporation of /sup 125/I and the immunoreactivity and stability of the labelled hormone. Employing highly purified monomeric hormone it proved possible, by the iodogen procedure, to prepare a labelled product of high specific activity irrespective of temperature. However, in radioiodinations performed at ambient temperature (20 to 25 degrees) significant amounts of the labelled hormone were in an aggregated form which was less immunoreactive than the /sup 125/I-labelled monomeric hormone. Such aggregation was largely prevented by radioiodinating at low temperature (0 to 4 degrees) and even the large monomeric peak was more immunoreactive (about 95% bound in antibody excess) than the monomeric peak from iodinations performed at room temperature (maximum binding 87%, or less).
- Research Organization:
- Department of Chemical Pathology, St. Bartholomew's Hospital, London, England
- OSTI ID:
- 6704785
- Journal Information:
- J. Immunoassay; (United States), Journal Name: J. Immunoassay; (United States) Vol. 2:3-4; ISSN JOUID
- Country of Publication:
- United States
- Language:
- English
Similar Records
Comparison of specific radioactivities of human alpha-lactalbumin iodinated by three different methods
Monoclonal antibody to growth hormone receptors
Preparation of /sup 125/I-labeled human growth hormone of high quality binding properties endowed with long-term stability
Journal Article
·
Wed Aug 01 00:00:00 EDT 1990
· Analytical Biochemistry; (USA)
·
OSTI ID:6172678
Monoclonal antibody to growth hormone receptors
Journal Article
·
Mon Dec 31 23:00:00 EST 1984
· Methods Enzymol.; (United States)
·
OSTI ID:6936362
Preparation of /sup 125/I-labeled human growth hormone of high quality binding properties endowed with long-term stability
Journal Article
·
Sat Dec 31 23:00:00 EST 1988
· J. Immunoassay; (United States)
·
OSTI ID:6075528
Related Subjects
550201 -- Biochemistry-- Tracer Techniques
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
BETA DECAY RADIOISOTOPES
CHEMICAL PREPARATION
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
HALOGENATION
HORMONES
INTERMEDIATE MASS NUCLEI
IODINATION
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LABELLING
NUCLEI
ODD-EVEN NUCLEI
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
RADIOPHARMACEUTICALS
STH
SYNTHESIS
TEMPERATURE EFFECTS
TRACER TECHNIQUES
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
BETA DECAY RADIOISOTOPES
CHEMICAL PREPARATION
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
HALOGENATION
HORMONES
INTERMEDIATE MASS NUCLEI
IODINATION
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LABELLING
NUCLEI
ODD-EVEN NUCLEI
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
RADIOPHARMACEUTICALS
STH
SYNTHESIS
TEMPERATURE EFFECTS
TRACER TECHNIQUES