Preparation of /sup 125/I-labeled human growth hormone of high quality binding properties endowed with long-term stability

Journal Article · Sun Jan 01 04:00:00 EST 1989 · J. Immunoassay; (United States)

DOI:https://doi.org/10.1080/01971528908053226· OSTI ID:6075528

Biscayart, P L; Paladini, A C; Vita, N; Roguin, L P

/sup 125/I-labeled human growth hormone (/sup 125/I-labeled.hGH) was prepared by using two variants of the chloramine T labelling procedure and purified by polyacrylamide gel electrophoresis (PAGE) of the reaction mixture. Variant A produced a tracer with high specific activity (100 +/- 10 microCi/microgram), high maximal binding capacity to antibodies (93%) and long-term stability (at least 150 days at -20/degree/C). No diiodinated tyrosil residues could be detected in this tracer. Variant B was devised to obtain higher yields of labeled hormone. The electrophoresis of the iodination mixture revealed two radioactive components with Rm values of 0.49 and 0.55 which result from the iodination of hGH variants preexisting in the starting material. Both tracers had similar specific activities (70 +/- 10 microCi/microgram), high maximal binding capacity to antibodies or receptors (80-100%, after 80 days of their obtention) and high stability (at least 100 days at -20/degree/C). It is concluded that the iododerivatives of hGH obtained by either method are adequate to perform radioimmunoassay and receptor studies and have long-term stability.

• Research Organization: Instituto de Quimica y Fisicoquimica Biologicas (UBA-CONICET), Buenos Aires (Argentina)
• OSTI ID: 6075528
• Journal Information: J. Immunoassay; (United States), Journal Name: J. Immunoassay; (United States) Vol. 10:1; ISSN JOUID
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CARBOXYLIC ACIDS
CHEMICAL PREPARATION
CHEMICAL PROPERTIES
CHLORAMINES
DAYS LIVING RADIOISOTOPES
DIIODOTYROSINE
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
HORMONES
HYDROXY ACIDS
IMMUNOASSAY
IMMUNOLOGY
IN VITRO
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MAN
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
PEPTIDE HORMONES
PITUITARY HORMONES
PRIMATES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
REACTION KINETICS
STABILITY
STH
SYNTHESIS
TRACER TECHNIQUES
TYROSINE
VERTEBRATES