Comparison of specific radioactivities of human alpha-lactalbumin iodinated by three different methods

Journal Article · Wed Aug 01 04:00:00 EDT 1990 · Analytical Biochemistry; (USA)

DOI:https://doi.org/10.1016/0003-2697(90)90615-G· OSTI ID:6172678

Thean, E T ^[1]

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1. Monash Medical School, Prahran, Victoria (Australia)

Radioiodination provides an extremely sensitive method for the detection of low levels of proteins. In the development of a sensitive radioimmunoassay for human alpha-lactalbumin (alpha-LA), the protein was labeled to high specific activity (approaching 2000 Ci/mmol) with lactoperoxidase, chloramine-T, and Iodogen. Despite high specific activities of the labeled protein by each method, there was a considerable difference in their binding affinity with monoclonal anti-human alpha-LA antibodies due to varying degrees of protein damage. Iodination of human alpha-LA with Iodogen resulted in labels of the highest specific activity and immunoreactivity with the monoclonal antibodies used.

• OSTI ID: 6172678
• Journal Information: Analytical Biochemistry; (USA), Journal Name: Analytical Biochemistry; (USA) Vol. 188:2; ISSN ANBCA; ISSN 0003-2697
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ANIMALS
ANTIBODIES
BIOASSAY
CHEMICAL REACTIONS
DIAGNOSTIC TECHNIQUES
ENZYMES
HALOGENATION
IMMUNOASSAY
IMMUNOLOGY
IODINATION
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MAN
MONOCLONAL ANTIBODIES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PEROXIDASES
PRIMATES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
TRACER TECHNIQUES
VERTEBRATES