Application of a monoclonal antibody to a comparative study of alpha-lactalbumins from various species

Journal Article · Mon May 01 04:00:00 EDT 1989 · J. Dairy Sci.; (United States)

DOI:https://doi.org/10.3168/jds.S0022-0302(89)79214-6· OSTI ID:5604069

Kaminogawa, S; Shimoda, M; Kurisaki, J; Yamauchi, K

A monoclonal antibody to bovine alpha-lactalbumin was prepared and purified. The binding ability of alpha-lactalbumin from different species (cow, goat, giraffe, horse, pig, human, monkey, and guinea pig) was examined by a competitive radioimmunoassay. The order in strength of the binding affinity was cow goat, giraffe, horse, cynomolgus monkey and human, pig, and guinea pig. The order of evolutional divergence calculated from the amino acid composition was cow, goat, giraffe, horse, pig, guinea pig and human, and monkey. The orders in both cases were similar. Hence, it is suggested that immunological divergence as deduced by a monoclonal antibody is likely to be close to the evolutional divergence of alpha-lactalbumin.

• Research Organization: Univ. of Tokyo (Japan)
• OSTI ID: 5604069
• Journal Information: J. Dairy Sci.; (United States), Journal Name: J. Dairy Sci.; (United States) Vol. 72:5; ISSN JDSCA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
AMINO ACIDS
ANIMALS
ANTIBODIES
BIOASSAY
BIOLOGICAL VARIABILITY
CARBOXYLIC ACIDS
CATTLE
COMPARATIVE EVALUATIONS
DOMESTIC ANIMALS
ENZYMES
GENETIC VARIABILITY
GUINEA PIGS
HORSES
IMMUNOASSAY
IMMUNOLOGY
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGASES
MAMMALS
MAN
MONKEYS
MONOCLONAL ANTIBODIES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RODENTS
RUMINANTS
SPECIFICITY
TRACER TECHNIQUES
VERTEBRATES