Monoclonal antibody to growth hormone receptors

Journal Article · Tue Jan 01 04:00:00 EST 1985 · Methods Enzymol.; (United States)

DOI:https://doi.org/10.1016/0076-6879(85)09124-8· OSTI ID:6936362

Simpson, J S.A.; Friesen, H G

Using hybridoma technology, monoclonal antibodies to growth hormone receptors can be prepared in large quantities with only a few micrograms of purified antigen by in vitro immunization or by immunization of larger quantities of less pure material. The discussion centers on areas most pertinent to receptors such as receptor preparation, immunization procedure, fusion method, screening assay, and identification of the immunoglobulin class. The specificity of the antibody for growth hormone receptor was examined by testing the ability of the ascitic fluid to inhibit binding of (/sup 125/I) growth hormone to the prolactin receptors on rabbit mammary gland membranes and the inhibition of /sup 125/I-labelled rat growth hormone binding to rabbit liver membrane.

• OSTI ID: 6936362
• Journal Information: Methods Enzymol.; (United States), Journal Name: Methods Enzymol.; (United States) Vol. 109; ISSN MENZA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIBODIES
ANTIBODY FORMATION
ANTIGENS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
DATA
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
EXPERIMENTAL DATA
GLANDS
GLOBULINS
HORMONES
HYBRIDOMAS
IMMUNOGLOBULINS
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LIVER
MAMMALS
MAMMARY GLANDS
MEMBRANE PROTEINS
MONOCLONAL ANTIBODIES
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PROTEINS
RABBITS
RADIOISOTOPES
RATS
REACTION KINETICS
RECEPTORS
RODENTS
TRACER TECHNIQUES
VERTEBRATES