Characterization of antisera to prolactin receptors

Journal Article · Tue Jan 01 04:00:00 EST 1985 · Methods Enzymol.; (United States)

DOI:https://doi.org/10.1016/0076-6879(85)09122-4· OSTI ID:6936381

Kelly, P A; Katoh, M; Djiane, J; Houdebine, L M; Dusanter-Fourt, I

Studies were conducted to improve the techniques of obtaining purified prolactin receptors and to immunize several species of animals with the partially purified receptor in order to increase the yield of antisera and to characterize the specific of these antisera in different target organs. Prolactin receptors were partially purified from crude microsomal fractions of lactating mammary glands, using affinity chromatography. For the assay of solubilized extracts of mammary gland samples at various stages of purification, /sup 125/I-labelled human growth hormone or ovine prolactin were used as labelled hormones. Antisera produced from partially purified prolactin receptors are able to inhibit binding of labelled hormone to its receptors in sera from sheep, goats, and guinea pigs. The antisera also inhibit the binding of prolactin to a number of tissues containing receptors in rabbits and rats.

• OSTI ID: 6936381
• Journal Information: Methods Enzymol.; (United States), Journal Name: Methods Enzymol.; (United States) Vol. 109; ISSN MENZA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
CELL CONSTITUENTS
CHROMATOGRAPHY
DATA
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
EXPERIMENTAL DATA
GLANDS
GOATS
GONADOTROPINS
GUINEA PIGS
HORMONES
IMMUNE SERUMS
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LTH
MAMMALS
MAMMARY GLANDS
MEMBRANE PROTEINS
MICROSOMES
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
PURIFICATION
RABBITS
RADIOISOTOPES
RATS
REACTION KINETICS
RECEPTORS
RODENTS
RUMINANTS
SEPARATION PROCESSES
SHEEP
TRACER TECHNIQUES
VERTEBRATES