Photoaffinity labeling of prolactin receptors

Journal Article · Tue Jan 01 04:00:00 EST 1985 · Methods Enzymol.; (United States)

DOI:https://doi.org/10.1016/0076-6879(85)09082-6· OSTI ID:6846128

Kelly, P A; Katoh, M; Djiane, J; Sakai, S

In order to elucidate the molecular characteristics of prolactin (PRL) receptors, a direct electrophoretic analysis of the relative molecular mass (M/sub r/) of purified PRL receptors and an electrophoretic analysis of photoaffinity-labelled receptors was utilized. Results of the study indicate that the major binding component in the membrane of rabbit mammary tissue has a M/sub r/ of 31,000-32,000 when affinity labelling combined with SDS-polyacrylamide gel electrophoresis were employed. Using electrophoresis under reducing conditions, a single M/sub r/ of 58,500 binding component-(/sup 125/I)PRL complex was obtained from rabbit mammary gland microsomes. Similar M/sub r/ binding components were also observed for other tissues from rabbits and pig mammary glands and slightly higher in M/sub r/ components from rat liver.

• OSTI ID: 6846128
• Journal Information: Methods Enzymol.; (United States), Journal Name: Methods Enzymol.; (United States) Vol. 109; ISSN MENZA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
DATA
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
EXPERIMENTAL DATA
GLANDS
GONADOTROPINS
HORMONES
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LABELLING
LIVER
LTH
MAMMALS
MAMMARY GLANDS
MEMBRANE PROTEINS
MOLECULAR WEIGHT
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
RABBITS
RADIOISOTOPES
RADIORECEPTOR ASSAY
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SWINE
TRACER TECHNIQUES
VERTEBRATES