Arachidonic acid metabolism in the platelets and neutrophils of diabetic rabbit and human subjects

Thesis/Dissertation · Mon Dec 31 23:00:00 EST 1984

OSTI ID:6703352

Greco, N J

An alteration of arachidonic acid metabolism to prostaglandins and leukotrienes from platelets and polymorphonuclear leukocytes respectively is evident in subjects with diabetes mellitus. There is evidence of altered platelet/vascular wall interactions in diabetes mellitus and evidence that polymorphonuclear leukocytes influence the vascular walls. Theories on the pathogenesis of atherosclerosis include both blood cells. Platelet hypersensitivity is evident in those platelets from the alloxan-induced diabetic rabbit either suspended in plasma or buffer. Arachidonic acid- and collagen-induced platelet aggregation, release of /sup 14/serotonin, and T x B/sub 2/ and 12-HETE production is enhanced when responses of diabetic platelets are compared to control platelets. Control rabbit neutrophils produce more LTB/sub 4/, LTB/sub 4/ isomers and 5-HETE than diabetic rabbits neutrophils. Decreased synthesis from diabetic rabbit neutrophils is not explained by increased catabolism of LTB/sub 4/, reesterification of 5-HETE, or increased eicosanoid formation. These experiments demonstrate both platelet and neutrophil dysfunction in diabetic subjects. Because of the involvement of these cells in regulating circulatory homeostatis, abnormal behavior could aggravate the atherosclerotic process. Platelet and neutrophil dysfunctions are noted before macroscopic vascular lesions are apparent suggesting an important role in the pathogenesis of atherosclerosis.

• Research Organization: Ohio State Univ., Columbus (USA)
• OSTI ID: 6703352
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMALS
ARACHIDONIC ACID
AROMATICS
ARTERIOSCLEROSIS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
AZOLES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
CHEMICAL ANALYSIS
CHROMATOGRAPHY
DIABETES MELLITUS
DISEASES
DRUGS
ENDOCRINE DISEASES
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INDOLES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKOCYTES
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MAN
MATERIALS
METABOLIC DISEASES
METABOLISM
MONOCARBOXYLIC ACIDS
NEUROREGULATORS
NEUTROPHILS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PROSTAGLANDINS
PYRROLES
QUANTITATIVE CHEMICAL ANALYSIS
RABBITS
RADIO-RELEASE ANALYSIS
RADIOPROTECTIVE SUBSTANCES
SEPARATION PROCESSES
SEROTONIN
SYMPATHOMIMETICS
TRACER TECHNIQUES
TRYPTAMINES
VASCULAR DISEASES
VERTEBRATES