Release of arachidonic acid from oligodendrocytes by terminal complement proteins, C5b-C9
Activation of C5b-C9 on monocytes, macrophages, platelets and neutrophils induces membrane lipid hydrolysis and generates arachidonic acid (AA) and its oxygenated derivatives. Additionally, activation of C5b-C9 and myelin lipid hydrolysis has been observed in demyelination. The authors have investigated the modulatory effect of C5b-9 on membrane lipid hydrolysis of oligodendrocytes (OLG), the myelin producing cells in the central nervous system. Antibody-sensitized rat OLG, prelabeled with /sup 14/C AA were treated with excess C6-deficient rabbit serum reconstituted with limiting doses of C6. Qualitative analysis of the supernatants by HPLC revealed the presence of both cyclooxygenase and lipooxygenase products. Prostaglandin E/sub 2/, leukotriene (LT) E/sub 4/, LTB/sub 4/ and free AA were the major radiolabeled products. The kinetics and dose response of LTB/sub 4/ release with respect to the cytolytic dose of C5b-9 were quantitated by radioimmunoassay. LTB/sub 4/ release approached maximum in 1 hr and higher amounts were detected with fewer C5b-9 channels. Addition of C8 to OLG bearing C5b-7 intermediates induced maximum LTB/sub 4/ release without further enhancement by C9 in contrast to the absolute requirement of C9 in mediator release from rat neutrophils. Thus, the requirement of C5b-8 or C5b-9 in mediator release appears to be cell-type dependent.
- Research Organization:
- Univ. of Maryland, Baltimore
- OSTI ID:
- 5453575
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 45:3; ISSN FEPRA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ARACHIDONIC ACID
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM
CHEMICAL REACTIONS
CHROMATOGRAPHY
COMPLEMENT
CONNECTIVE TISSUE CELLS
DECOMPOSITION
DOSE-RESPONSE RELATIONSHIPS
ENZYMES
HYDROLYSIS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LEUKOCYTES
LIPIDS
LIQUID COLUMN CHROMATOGRAPHY
LYSIS
MACROPHAGES
MATERIALS
MEMBRANES
MONOCARBOXYLIC ACIDS
NERVOUS SYSTEM
NEUTROPHILS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
PHAGOCYTES
PROSTAGLANDINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
REACTION KINETICS
SECRETION
SEPARATION PROCESSES
SOLVOLYSIS
SOMATIC CELLS
TRACER TECHNIQUES