Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Multimeric C9 within C5b-9 deposits in unique locations in the cell wall of Salmonella typhimurium

Journal Article · · J. Immunol.; (United States)
OSTI ID:5934653
; ; ;

We have shown previously that multimeric C9 within C5b-9 (C9:C5b-8 greater than 3:1) is needed for killing of a rough strain of Escherichia coli. We now extend these studies using serum sensitive, rough (R) and serum resistant, wild type (WT) strains of Salmonella typhimurium as well as a mutant S. typhimurium strain (TS) with a temperature sensitive mutation in synthesis of keto-deoxy-octulosonate, a constituent within the deep core structure of Salmonella LPS. Both R and TS required multimeric C9 within C5b-9 to be killed. Addition at 37 degrees C of increasing inputs of C9 to TS or R bearing C5b-9 led to a dose-related increase in C9 binding and killing. In contrast, addition of high inputs of C9 to the same strains at 4 degrees C, a procedure that limits the C9:C5b-8 ratio to 1:1, resulted in low C9 binding and minimal killing. Bactericidal C5b-9 formed at 37 degrees C on R and TS with high inputs of C9 co-sedimented with the bacterial outer membrane on sucrose density gradient analysis. Non-bactericidal C5b-9 on R, WT, and TS co-sedimented near the inner membrane, despite the presumed lack of association between these constituents. Whereas /sup 125/I C9 within the non-bactericidal pools immunoprecipitate with anti-C5, /sup 125/I C9 within bactericidal pools did not immunoprecipitate with anti-C5, anti-C7, or anti-C9. These findings suggest that bactericidal C5b-9 may be deposited in a unique location or configuration within the bacterial cell wall.

Research Organization:
National Institute of Allergy and Infectious Diseases, Bethesda, MD (USA)
OSTI ID:
5934653
Journal Information:
J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 142:12; ISSN JOIMA
Country of Publication:
United States
Language:
English

Similar Records

Multimeric complement component C9 is necessary for killing of Escherichia coli J5 by terminal attack complex C5b-9
Journal Article · Mon Jul 01 00:00:00 EDT 1985 · Proc. Natl. Acad. Sci. U.S.A.; (United States) · OSTI ID:5095456
Release of arachidonic acid from oligodendrocytes by terminal complement proteins, C5b-C9
Conference · Fri Feb 28 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) · OSTI ID:5453575
Killing of human melanoma cells by the membrane attack complex (MAC) as a function of its molecular composition
Conference · Fri Feb 28 23:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) · OSTI ID:5495592

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BACTERIA
BETA DECAY RADIOISOTOPES
BIOASSAY
BIOLOGICAL LOCALIZATION
CELL CONSTITUENTS
CELL KILLING
CELL WALL
CENTRIFUGATION
COMPLEMENT
DAYS LIVING RADIOISOTOPES
DOSE-RESPONSE RELATIONSHIPS
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
IMMUNOASSAY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
MAMMALS
MAN
MEMBRANE PROTEINS
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RADIOISOTOPES
RECEPTORS
SALMONELLA
SALMONELLA TYPHIMURIUM
SEPARATION PROCESSES
TEMPERATURE DEPENDENCE
VERTEBRATES