In vitro activation of suppressor cells from spleens of mice treated with radioactive strontium. [/sup 89/Sr]

Journal Article · Tue Aug 01 04:00:00 EDT 1978 · J. Immunol.; (United States)

OSTI ID:6698419

Merluzzi, V J; Levy, E M; Kumar, V; Bennett, M; Cooperband, S R

Mice were treated with two 100-..mu..Ci injections of /sup 89/Sr to deplete marrow-dependent (M) cells. Mice so treated responded normally to immunization with sheep red blood cells (SRBC) in vivo; moreover, spleen cells from /sup 89/Sr-treated mice were able to respond to SRBC after infusion into irradiated recipient mice. However, spleen cells from mice treated with /sup 89/Sr did not respond to SRBC in vitro and mixtures of normal spleen cells with the latter were also not able to respond in vitro. The discrepancy between in vivo and in vitro responses was abolished by culturing spleen cells for 24 hr before testing their ability to respond to SRBC in the adoptive transfer in vivo. Pretreatment of spleen cells from /sup 89/Sr-treated mice with 1000 R of ..gamma.. radiation lessened their suppressive activity. The suppressor cells were detected in spleens of athymic nude mice treated with /sup 89/Sr. The suppressive activity, after the 24-hr culture period, was not abolished by irradiation and was active in vivo as well as in vitro. Thus, depletion of M cells by /sup 89/Sr results in the appearance within the spleen of thymus-independent suppressor cells, which require a short period of in vitro cultivation before becoming functionally active.

• Research Organization: Boston Univ. School of Medicine
• OSTI ID: 6698419
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 121:2; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

560162* -- Radionuclide Effects
Kinetics
& Toxicology-- Animals
Plants
Microorganisms
& Cells
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH ISOTOPES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
BONE MARROW
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
HEMATOPOIETIC SYSTEM
IMMUNE REACTIONS
IMMUNOSUPPRESSION
IN VITRO
INHIBITION
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
INTERNAL IRRADIATION
INTRAPERITONEAL INJECTION
IRRADIATION
ISOTOPES
MAMMALS
MICE
NUCLEI
ORGANS
RADIATION EFFECTS
RADIOINDUCTION
RADIOISOTOPES
RODENTS
SOMATIC CELLS
SPLEEN CELLS
STRONTIUM 89
STRONTIUM ISOTOPES
TISSUES
VERTEBRATES