Natural killer cells in mice treated with /sup 89/Strontium: normal target-binding cell numbers but inability to kill even after interferon administration

Journal Article · Mon Oct 01 00:00:00 EDT 1979 · J. Immunol.; (United States)

OSTI ID:5299137

Kumar, V; Ben-Ezra, J; Bennett, M; Sonnenfeld, G

Natural Killer (NK) cells activity against YAC-1 lymphoma cells is greatly reduced in spleens of mice injected previously with the bone-seeking isotope, /sup 89/Sr. Spleen cells from /sup 89/Sr-treated mice filtered over nylon wool and Sephadex G-10 columns failed to increase the low NK cell activity. Cell mixture experiments failed to show inhibition of NK activity of normal spleen cells by spleen cells from /sup 89/Sr-treated mice. /sup 89/Sr produced lowering of NK activity in athymic nude mice similar to that seen in control mice. These experiments suggest that the low NK activity in /sup 89/Sr-treated mice is not due to the presence of thymus-dependent or independent suppressor cells active at the effector phase of NK cell-mediated killing. The low NK activity of /sup 89/Sr-treated mice could not be stimulated by administration of interferon inducers, polyinosinic:polycytidylic acid and Corynebacterium parvum, or by preparations containing type I or II interferons. The frequency of cells capable of binding to YAC-1 target cells is normal in spleens of /sup 89/Sr-treated mice. These data suggest that in /sup 89/Sr-treated mice, NK cells exist in a state in which they can bind the target cells but cannot lyse them and are unresponsive to the activating effect of interferons. Transformation of these cells into fully lytic and interferon-responsive cells appears to require an intact bone marrow. Bone marrow cell infusions failed to restore NK activity in irradiated or unirradiated /sup 89/Sr-treated mice, but were able to do so in normal lethally irradiated mice. Thus functional NK cells reactive against YAC-1 lymphoma cells are not only marrow derived but also marrow dependent.

• Research Organization: Boston Univ., MA
• OSTI ID: 5299137
• Journal Information: J. Immunol.; (United States), Vol. 123:4
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
BONE MARROW CELLS
IMMUNE REACTIONS
RADIONUCLIDE KINETICS
BIOLOGICAL RADIATION EFFECTS
INTERFERON
RADIOSENSITIVITY EFFECTS
BONE MARROW
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IMMUNITY
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SPLEEN CELLS
STEM CELLS
STRONTIUM 89
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ALKALINE EARTH ISOTOPES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
EVEN-ODD NUCLEI
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INTERMEDIATE MASS NUCLEI
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MAMMALS
NUCLEI
ORGANS
RADIATION EFFECTS
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SOMATIC CELLS
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TISSUES
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