Suppression of cytotoxic T lymphocytes by carrageenan-activated macrophage-like cells. [Gamma radiation]
In the presence of 100 ..mu..g/ml of carrageenans (CAR), B6D2F/sub 1/ responder spleen cells failed to generate antiparent or anti-allogeneic cytotoxic T lymphocytes in vitro, but instead generated suppressor cells. Cultured CAR-treated cells added to mixtures of B6D2F/sub 1/ anti-B6 or B6D2F/sub 1/ anti-C3H cytotoxic effectors (induced in vitro) and the appropriate /sup 51/Cr-labeled lymphoma targets reduced or abolished cytolysis (measured as /sup 51/Cr release) depending on the ratio of suppressor to effector cells. Cultured spleen cells not exposed to CAR failed to inhibit both types of cytotoxicity. Presuppressor cells were associated with a splenic subpopulation independent of the thymus (i.e., present in spleens of athymic nude mice), were moderately adherent to Sephadex G-10 columns, but were not phagocytic or ''sticky'' to carbonyl iron particles. Activation of such cells by CAR was not prevented by in vitro exposure to 2000 rads of ..gamma..-rays before culture, nor facilitated by antigenic stimulation. The matured suppressor cells remained radioresistant and became strongly adherent to Sephadex G-10. The suppressors lacked surface Thy-1 alloantigen detectable by antibody and rabbit complement. Suppressor cell activity was not restricted by the immunologic specificity and major histocompatibility type of effectors.
- Research Organization:
- State Univ. of New York, Buffalo
- OSTI ID:
- 6469845
- Journal Information:
- J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 121:5; ISSN JOIMA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CELL CULTURES
CHEMICAL ACTIVATION
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
IMMUNE REACTIONS
IONIZING RADIATIONS
LEUKOCYTES
LYMPHOCYTES
ORGANIC COMPOUNDS
POLYSACCHARIDES
RADIATION EFFECTS
RADIATIONS
SACCHARIDES
SOMATIC CELLS
SPLEEN CELLS
TOXICITY