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Bone marrow cytotoxic precursor T cells: alloantigen-induced cytotoxic T-cell responses by murine bone marrow cells in vitro

Journal Article · · Cell. Immunol.; (United States)
; ; ;

When bone marrow cells, obtained from C57BL/6 mice, were incubated in vitro with irradiated BALB/c spleen cells, cytotoxic activity against P815 tumor cells developed at 5 to 7 days of culture. This cytotoxic activity was T cell mediated and specific for the H-2 type of the allogeneic spleen cells used as stimulators. The generation of this cytotoxic T-cell activity was dependent on T cells present in the bone marrow since T-cell-depleted bone marrow cells failed to generate a cytotoxic response in vitro. Further, bone marrow cells incubated in culture medium not supplemented with 2-mercaptoethanol (2-ME) failed to generate alloantigen induced cytotoxic responses. Cytotoxic activity generated by bone marrow cells was always lower than the cytotoxic activity generated by alloantigen stimulated spleen cells. The possibility that differentiation of pre-T cells into cytotoxic effector T cells is taking place in this in vitro response is considered.

Research Organization:
Univ. of Washington, Seattle
OSTI ID:
6338068
Journal Information:
Cell. Immunol.; (United States), Journal Name: Cell. Immunol.; (United States) Vol. 61:1; ISSN CLIMB
Country of Publication:
United States
Language:
English

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Related Subjects

550301* -- Cytology-- Tracer Techniques
551001 -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
BONE MARROW CELLS
CHROMIUM 51
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
IMMUNITY
IMMUNOLOGY
IN VITRO
INTERMEDIATE MASS NUCLEI
ISOTOPES
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
METABOLIC ACTIVATION
MICE
NUCLEI
RADIOISOTOPES
RODENTS
SOMATIC CELLS
SPLEEN CELLS
TOXICITY
TUMOR CELLS
VERTEBRATES