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Natural regulatory cells in murine bone marrow: inhibition of in vitro proliferative and cytotoxic responses to alloantigens

Journal Article · · J. Immunol.; (United States)
OSTI ID:5729377
; ;

A lymphocyte-enriched fraction of murine bone marrow (BML), obtained by sucrose density fractionation, contains natural regulatory cells that can profoundly suppress the proliferative and cytotoxic response of syngeneic lymph node cells to irradiated alloantigens in a mixed lymphocyte culture (MLC). A close correlation exists between the inhibition of alloantigen-induced proliferation and the generation of cytotoxic effectors. The suppression of proliferation is dependent on the dose of BML added to the cultures but is not due to cell crowding. The addition of BML to cultures does not cause the maximum proliferative response to change from the usual day 5 peak, indicating that there is no change in culture kinetics. The release of nonradioactive thymidine by BML cannot explain the suppression. The target of suppression is maximally affected during the first 24 h of culture, since adding BML to MLC later than this resulted in negligible inhibition of proliferation. Thus, the natural regulatory cell-mediated suppression reflects inhibition of earlyevents in the proliferative response to alloantigens.

Research Organization:
Univ. of Washington, Seattle
DOE Contract Number:
EY-76-S-06-2225
OSTI ID:
5729377
Journal Information:
J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 124:6; ISSN JOIMA
Country of Publication:
United States
Language:
English

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Related Subjects

550301* -- Cytology-- Tracer Techniques
551001 -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
BONE MARROW CELLS
CELL PROLIFERATION
CHROMIUM 51
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
IMMUNE REACTIONS
IMMUNOSUPPRESSION
IN VITRO
INHIBITION
INTERMEDIATE MASS NUCLEI
IONIZING RADIATIONS
ISOTOPES
LABELLED COMPOUNDS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
NUCLEI
RADIATIONS
RADIOASSAY
RADIOISOTOPES
RODENTS
SOMATIC CELLS
TRITIUM COMPOUNDS
TUMOR CELLS
VERTEBRATES