Sister chromatid exchange induced by X-irradiation of retinoblastoma lymphocytes

Journal Article · Fri Jun 01 04:00:00 EDT 1984 · Invest. Ophthalmol. Visual Sci.; (United States)

OSTI ID:6661669

Abramovsky-Kaplan, I; Jones, I S

Lymphocyte cultures were employed to assess the degree of spontaneous and induced chromosomal fragility in retinoblastoma. Sister chromatid exchange (SCEs) were scored in metaphases. Three unilateral, three bilateral, eleven family members and controls were studied. Retinoblastoma (RB) lymphocytes did not exhibit increased spontaneous fragility. X-irradiation (25-200 rad) did not significantly increase SCE in unilateral retinoblastoma lymphocytes when compared with controls (P greater than 0.50). However, bilaterally affected subjects and three unaffected relatives demonstrated a statistically significant increase in SCE (P less than 0.01). In conclusion, hereditary retinoblastoma lymphocytes appear more radiosensitive than sporadic retinoblastoma, perhaps, reflecting the increased second malignancies in germinal mutation retinoblastoma. In addition, the analysis of radiation-induced SCE in peripheral blood lymphocytes of RB patients and family members may provide a valuable tool increasing the accuracy of genetic counseling for this disorder. Additional studies of RB patients and families are needed to assess the relevance of this approach to genetic counseling.

• Research Organization: Edward S. Harkness Eye Institute, Columbia Presbyterian Medical Center, New York, New York
• OSTI ID: 6661669
• Journal Information: Invest. Ophthalmol. Visual Sci.; (United States), Journal Name: Invest. Ophthalmol. Visual Sci.; (United States) Vol. 25:6; ISSN IOVSD
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY AREAS
BODY FLUIDS
CARCINOMAS
CELL CULTURES
CHROMOSOMAL ABERRATIONS
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROMAGNETIC RADIATION
EYES
FACE
GERM CELLS
HEAD
HEREDITARY DISEASES
IONIZING RADIATIONS
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MUTATIONS
NEOPLASMS
ORGANS
RADIATIONS
RADIOSENSITIVITY
RETINA
SENSE ORGANS
SISTER CHROMATID EXCHANGES
SOMATIC CELLS
TUMOR CELLS
X RADIATION