Sister chromatid exchanges in human lymphocytes exposed to ionizing radiation during G/sub 0/. [. gamma. rays]

Journal Article · Fri Jun 01 04:00:00 EDT 1979 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3574976· OSTI ID:5993950

Littlefield, L G; Colyer, S P; Joiner, E E; DuFrain, R J

Several authors have reported small, but statistically significant, increases in sister chromatid exchanges (SCEs) in mammalian cells exposed to low-LET radiation during culture in the presence of (/sup 3/H)-thymidine or bromodeoxyuiridine (BUdR). To determine whether ionizing radiation also induces lesions in the unsubstituted DNA of G/sub 0/ lymphocytes that lead to SCEs in second division metaphases, we exposed human lymphocytes to 150 or 300 /sup 60/Co-..gamma.. radiation prior to culture in the presence of phytohemagglutinin and BUdR. Increased frequencies of SCEs were not observed in the irradiated cultures, either in second division metaphases having ring and dicentric chromosomes or those that did not have detectable chromosome aberrations. these findings suggest that increased frequencies of SCEs would not be observed in cultured lymphocytes from persons with in vivo exposures to radiation, and raise questions regarding the efficacy of ionizing radiation in inducing long-lived lesions in native DNA that can subsequently be expressed as SCEs.

• Research Organization: Oak Ridge Associated Universities, TN
• OSTI ID: 5993950
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 78:3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CULTURES
CELL CYCLE
CHROMATIDS
COBALT 60
COBALT ISOTOPES
CONNECTIVE TISSUE CELLS
DATA
DATA FORMS
DNA
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
GAMMA RADIATION
IN VITRO
INFORMATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
IRRADIATION
ISOLATED VALUES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MINUTES LIVING RADIOISOTOPES
MUTATIONS
NUCLEI
NUCLEIC ACIDS
NUMERICAL DATA
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PRIMATES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
RADIOSENSITIVITY
SISTER CHROMATID EXCHANGES
SOMATIC CELLS
VERTEBRATES
YEARS LIVING RADIOISOTOPES