Direct evidence for the role of incorporated BUdR in the induction of sister chromatid exchanges
Journal Article
·
· Exp. Cell Res.; (United States)
The level of bromodeoxyuridine (BUdR) substitution for thymidine (dThd) in nascent DNA of Chinese hamster cells increases rapidly with increasing BUdR concentration at low BUdR/base pair (BU/bp) ratios and then increases much more slowly at higher ratios. The relationship between percentage substitution and BU/bp indicates that BUdR competes with dThd for incorporation into DNA and that BUdR concentration has no effect on the effective dThd/base pair ratio in these cells. Equal amounts of BUdR are incorporated during both the first and second cell cycles (S phases) after provision of the dThd analog. Incorporation of BUdR induces sister chromatid exchanges (SCEs), and induction appears to occur during the cell cycle (S phase) in which incorporation occurs, with incorporated BUdR from previous cycles having little effect. BUdR-induced SCE frequencies are linearly proportional to the percentage substitution of BUdR for dThd. Extrapolation to zero substitution indicates that SCEs occur spontaneously at a frequency of 0.045 per chromosome per cycle, and that many so-called spontaneous SCEs are actually induced by BUdR.
- Research Organization:
- Univ. of California, Livermore
- OSTI ID:
- 5368354
- Journal Information:
- Exp. Cell Res.; (United States), Journal Name: Exp. Cell Res.; (United States) Vol. 117; ISSN ECREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550400 -- Genetics
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTIMETABOLITES
ANTIMITOTIC DRUGS
AZINES
BIOLOGICAL EFFECTS
BROMOURACILS
BUDR
CELL CULTURES
CELL CYCLE
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
DNA
DOSE-RESPONSE RELATIONSHIPS
DRUGS
GENETIC EFFECTS
HAMSTERS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
MAMMALS
MUTATIONS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RIBOSIDES
RODENTS
SISTER CHROMATID EXCHANGES
THYMIDINE
TOXICITY
URACILS
VERTEBRATES
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTIMETABOLITES
ANTIMITOTIC DRUGS
AZINES
BIOLOGICAL EFFECTS
BROMOURACILS
BUDR
CELL CULTURES
CELL CYCLE
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
DNA
DOSE-RESPONSE RELATIONSHIPS
DRUGS
GENETIC EFFECTS
HAMSTERS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
MAMMALS
MUTATIONS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RIBOSIDES
RODENTS
SISTER CHROMATID EXCHANGES
THYMIDINE
TOXICITY
URACILS
VERTEBRATES