Different effects of mutagens on sister chromatid exchange induction in three Chinese hamster cell lines

Journal Article · Sun Jan 01 04:00:00 EST 1984 · Environ. Mutagen.; (United States)

DOI:https://doi.org/10.1002/em.2860060410· OSTI ID:6220469

Mehnert, K; Vogel, W; Benz, R; Speit, G

The sister chromatid exchange (SCE) induction of mutgens with different mechanisms of action was comparatively investigated on permanent cell lines of the Chinese hamster (CHO, V-79, and DON) with and without exogenous metabolic activation and with the use of various experimental protocols. CHO and V-79 cells were treated with ethylmethanesulfonate (EMS), a direct mutagen; with the two indirect mutagens cyclophosphamide (CP) and benzo(a)pyrene (BP); as well as with the radical former hydrogen peroxide (H/sub 2/O/sub 2/) and hydroxyurea (HU), an inhibitor of DNA synthesis. Aside from an increased basal SCE level and a higher bromodeoxyuridine (BrdUrd) sensitivity, there was no decisive difference between CHO and V-79 cells. Neither cell line was able to metabolize the indirect mutagen BP. Only in CHO cells did CP lead to increased SCE frequencies. However, in all cases, the simultaneous application of S9 mix produced a distinct SCE induction. In contrast, BP caused SCE induction in DON cells, whereas CP was not metabolized. The results indicate that closely related cell lines differ in their capability for inducing SCE and that investigations of SCE inductions performed on only one cell line do not necessarily produce a representative response.

• Research Organization: Universitaet Ulm, West Germany
• OSTI ID: 6220469
• Journal Information: Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 6:4; ISSN ENMUD
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
AMIDES
ANIMAL CELLS
ANIMALS
AROMATICS
BENZOPYRENE
BIOASSAY
BIOLOGICAL EFFECTS
CHO CELLS
CHROMOSOMAL ABERRATIONS
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
DATA
DRUGS
EMS
ENDOXAN
ESTERS
EXPERIMENTAL DATA
FIBROBLASTS
GENETIC EFFECTS
HAMSTERS
HYDROCARBONS
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
HYDROXY COMPOUNDS
HYDROXYUREA
IMMUNOSUPPRESSIVE DRUGS
INFORMATION
MAMMALS
METABOLIC ACTIVATION
MUTAGENS
MUTATIONS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXYGEN COMPOUNDS
PEROXIDES
RODENTS
SISTER CHROMATID EXCHANGES
SOMATIC CELLS
SULFONIC ACID ESTERS
VERTEBRATES