[Tonoplast transport and salt tolerance in plants]
We have showed that the tonoplast V-ATPase could be specifically inhibited by antisense DNA to the catalytic (A) subunit; that cell expansion was inhibited in carrot transformants deficient in the enzyme and have provided evidence for at least two different isoforms of the A subunit which are Golgi- and tonoplast-specific. These findings prompted a search for sequences of the isoforms of the A subunit in carrot. We have cloned and sequenced 1.0--1.5 kb fragments of three different genes for the catalytic subunit, the fragments differ greatly in their introns, but have nearly identical exons. We are using PCR to amplify and subclone carrot seedling cDNA. Thus far two bands have been amplified and are currently being subcloned for sequencing.
- Research Organization:
- California Univ., Santa Cruz, CA (United States)
- Sponsoring Organization:
- DOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG03-84ER13245
- OSTI ID:
- 6653558
- Report Number(s):
- DOE/ER/13245-T2; ON: DE93011924
- Country of Publication:
- United States
- Language:
- English
Similar Records
[Tonoplast transport and salt tolerance in plants]. Progress report
Regulation of the synthesis and assembly of the plant vacuolar H sup + -ATPase
Regulation of the synthesis and assembly of the plant vacuolar H{sup +}-ATPase. Progress report, [April 1, 1991--March 31, 1992]
Technical Report
·
Wed Mar 31 23:00:00 EST 1993
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OSTI ID:10141769
Regulation of the synthesis and assembly of the plant vacuolar H sup + -ATPase
Technical Report
·
Tue Dec 31 23:00:00 EST 1991
·
OSTI ID:5618759
Regulation of the synthesis and assembly of the plant vacuolar H{sup +}-ATPase. Progress report, [April 1, 1991--March 31, 1992]
Technical Report
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Tue Mar 31 23:00:00 EST 1992
·
OSTI ID:10135025
Related Subjects
09 BIOMASS FUELS
090700 -- Biomass Fuels-- Resources-- (1990-)
090800 -- Biomass Fuels-- Production-- (1990-)
550200* -- Biochemistry
553000 -- Agriculture & Food Technology
59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
ACID ANHYDRASES
ALKALI METAL COMPOUNDS
ARABIDOPSIS
ATP-ASE
BIOLOGICAL ADAPTATION
CARROTS
CHLORIDES
CHLORINE COMPOUNDS
CLONING
COSMIDS
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
DOCUMENT TYPES
ENZYMES
FOOD
HALIDES
HALOGEN COMPOUNDS
HYBRIDIZATION
HYDROLASES
IRRIGATION
MAGNOLIOPHYTA
MAGNOLIOPSIDA
ORGANIC COMPOUNDS
PHOSPHOHYDROLASES
PLANTS
PROGRESS REPORT
PROTEIN STRUCTURE
PROTEINS
SODIUM CHLORIDES
SODIUM COMPOUNDS
STRUCTURAL CHEMICAL ANALYSIS
VEGETABLES
090700 -- Biomass Fuels-- Resources-- (1990-)
090800 -- Biomass Fuels-- Production-- (1990-)
550200* -- Biochemistry
553000 -- Agriculture & Food Technology
59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
ACID ANHYDRASES
ALKALI METAL COMPOUNDS
ARABIDOPSIS
ATP-ASE
BIOLOGICAL ADAPTATION
CARROTS
CHLORIDES
CHLORINE COMPOUNDS
CLONING
COSMIDS
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
DOCUMENT TYPES
ENZYMES
FOOD
HALIDES
HALOGEN COMPOUNDS
HYBRIDIZATION
HYDROLASES
IRRIGATION
MAGNOLIOPHYTA
MAGNOLIOPSIDA
ORGANIC COMPOUNDS
PHOSPHOHYDROLASES
PLANTS
PROGRESS REPORT
PROTEIN STRUCTURE
PROTEINS
SODIUM CHLORIDES
SODIUM COMPOUNDS
STRUCTURAL CHEMICAL ANALYSIS
VEGETABLES