[Tonoplast transport and salt tolerance in plants]. Progress report

Technical Report · Thu Apr 01 04:00:00 EST 1993

DOI:https://doi.org/10.2172/10141769· OSTI ID:10141769

Taiz, L

We have showed that the tonoplast V-ATPase could be specifically inhibited by antisense DNA to the catalytic (A) subunit; that cell expansion was inhibited in carrot transformants deficient in the enzyme and have provided evidence for at least two different isoforms of the A subunit which are Golgi- and tonoplast-specific. These findings prompted a search for sequences of the isoforms of the A subunit in carrot. We have cloned and sequenced 1.0--1.5 kb fragments of three different genes for the catalytic subunit, the fragments differ greatly in their introns, but have nearly identical exons. We are using PCR to amplify and subclone carrot seedling cDNA. Thus far two bands have been amplified and are currently being subcloned for sequencing.

• Research Organization: California Univ., Santa Cruz, CA (United States)
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: FG03-84ER13245
• OSTI ID: 10141769
• Report Number(s): DOE/ER/13245--T2; ON: DE93011924
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
090700
090800
550200
553000
59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
AGRICULTURE AND FOOD TECHNOLOGY
ARABIDOPSIS
ATP-ASE
BIOCHEMISTRY
BIOLOGICAL ADAPTATION
CARROTS
COSMIDS
DNA SEQUENCING
DNA-CLONING
IRRIGATION
PRODUCTION
PROGRESS REPORT
PROTEIN STRUCTURE
RESOURCES
SODIUM CHLORIDES