[Tonoplast transport and salt tolerance in plants]. Progress report
We have showed that the tonoplast V-ATPase could be specifically inhibited by antisense DNA to the catalytic (A) subunit; that cell expansion was inhibited in carrot transformants deficient in the enzyme and have provided evidence for at least two different isoforms of the A subunit which are Golgi- and tonoplast-specific. These findings prompted a search for sequences of the isoforms of the A subunit in carrot. We have cloned and sequenced 1.0--1.5 kb fragments of three different genes for the catalytic subunit, the fragments differ greatly in their introns, but have nearly identical exons. We are using PCR to amplify and subclone carrot seedling cDNA. Thus far two bands have been amplified and are currently being subcloned for sequencing.
- Research Organization:
- California Univ., Santa Cruz, CA (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG03-84ER13245
- OSTI ID:
- 10141769
- Report Number(s):
- DOE/ER/13245-T2; ON: DE93011924
- Resource Relation:
- Other Information: PBD: [1993]
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
09 BIOMASS FUELS
60 APPLIED LIFE SCIENCES
CARROTS
BIOLOGICAL ADAPTATION
ATP-ASE
DNA-CLONING
DNA SEQUENCING
IRRIGATION
SODIUM CHLORIDES
ARABIDOPSIS
COSMIDS
PROTEIN STRUCTURE
PROGRESS REPORT
550200
090800
090700
553000
BIOCHEMISTRY
PRODUCTION
RESOURCES
AGRICULTURE AND FOOD TECHNOLOGY