Regulation of the synthesis and assembly of the plant vacuolar H sup + -ATPase

Technical Report · Wed Jan 01 04:00:00 EST 1992

DOI:https://doi.org/10.2172/5618759· OSTI ID:5618759

Taiz, L

During the past three years we have focused on four main areas: the characterization of the 5{prime}-upstream sequence of the gene for the V-ATPase 70 kDa (A) subunit gene, the generation of V-ATPase-deficient mutants using antisense constructs of the A subunit cDNA, analysis of V-ATPase ultrastructure by negative staining and the characterization of organelle-specific isoforms of the A subunit of carrot. In addition we have extended our studies on the cellular distribution of the V-ATPase and we have continued our investigation of the evolution of the V-ATPases by characterizing the A and B subunits of two species of the archaebacterium, Methanococcus.

• Research Organization: California Univ., Santa Cruz, CA (United States). Dept. of Biology
• Sponsoring Organization: DOE; USDOE, Washington, DC (United States)
• DOE Contract Number: FG03-84ER13245
• OSTI ID: 5618759
• Report Number(s): DOE/ER/13245-T1; ON: DE92010627
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ACID ANHYDRASES
ATP-ASE
BACTERIA
CARROTS
CELL CONSTITUENTS
CLONING
DISTRIBUTION
DNA SEQUENCING
DOCUMENT TYPES
ENZYMES
FOOD
HYDROLASES
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MICROORGANISMS
MITOCHONDRIA
MUTATIONS
ORGANIC COMPOUNDS
PHOSPHOHYDROLASES
PLANTS
PROGRESS REPORT
PROTEINS
RESEARCH PROGRAMS
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
VEGETABLES