[Tonoplast transport and salt tolerance in plants]

Technical Report · Fri Jan 01 00:00:00 EST 1993

DOI:https://doi.org/10.2172/6653558· OSTI ID:6653558

Taiz, L

We have showed that the tonoplast V-ATPase could be specifically inhibited by antisense DNA to the catalytic (A) subunit; that cell expansion was inhibited in carrot transformants deficient in the enzyme and have provided evidence for at least two different isoforms of the A subunit which are Golgi- and tonoplast-specific. These findings prompted a search for sequences of the isoforms of the A subunit in carrot. We have cloned and sequenced 1.0--1.5 kb fragments of three different genes for the catalytic subunit, the fragments differ greatly in their introns, but have nearly identical exons. We are using PCR to amplify and subclone carrot seedling cDNA. Thus far two bands have been amplified and are currently being subcloned for sequencing.

• Research Organization: California Univ., Santa Cruz, CA (United States)
• Sponsoring Organization: USDOE; USDOE, Washington, DC (United States)
• DOE Contract Number: FG03-84ER13245
• OSTI ID: 6653558
• Report Number(s): DOE/ER/13245-T2; ON: DE93011924
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
09 BIOMASS FUELS
60 APPLIED LIFE SCIENCES
ATP-ASE
DNA SEQUENCING
DNA-CLONING
CARROTS
BIOLOGICAL ADAPTATION
ARABIDOPSIS
COSMIDS
IRRIGATION
PROGRESS REPORT
PROTEIN STRUCTURE
SODIUM CHLORIDES
ACID ANHYDRASES
ALKALI METAL COMPOUNDS
CHLORIDES
CHLORINE COMPOUNDS
CLONING
DNA HYBRIDIZATION
DOCUMENT TYPES
ENZYMES
FOOD
HALIDES
HALOGEN COMPOUNDS
HYBRIDIZATION
HYDROLASES
MAGNOLIOPHYTA
MAGNOLIOPSIDA
ORGANIC COMPOUNDS
PHOSPHOHYDROLASES
PLANTS
PROTEINS
SODIUM COMPOUNDS
STRUCTURAL CHEMICAL ANALYSIS
VEGETABLES
550200* - Biochemistry
090800 - Biomass Fuels- Production- (1990-)
090700 - Biomass Fuels- Resources- (1990-)
553000 - Agriculture & Food Technology