Fate of circulating renin in conscious rats
Highly purified /sup 125/I-labeled rat renal renin (/sup 125/I-renin) was given intravenously to conscious rats to study the fate of circulating renin. Specific antirat renin antiserum was used to identify the labeled renin molecules. In sham-operated rats, the disappearance of /sup 125/I-renin from the plasma showed two exponential components with a half-life of 6.7 +/- 0.4 min for the rapid component and 65.1 +/- 5.7 min for the slow component. The metabolic clearance rate was 11.4 +/- 1.0 ml X min-1 X kg-1. In bilaterally nephrectomized rats, the metabolic clearance rate of /sup 125/I-renin was reduced by 55%, but the half-life of the slow component remained unchanged. Seventy percent hepatectomy caused a 54% decrement in the metabolic clearance and prolonged the half-life of the slow component. Five minutes after injection of /sup 125/I-renin, approximately 59 and 11% of the administered /sup 125/I-renin had accumulated in the liver and the kidneys, respectively, and at later time points the /sup 125/I-renin was highly concentrated in these organs. High-performance liquid chromatographic analysis of the liver and kidney extracts demonstrated that /sup 125/I-renin was catabolized by these organs. Biliary excretion of /sup 125/I-renin was negligible. Urinary excretion of /sup 125/I-renin up to 120 min was approximately 2% of the injected dose. We conclude that both the liver and the kidney are responsible for the clearance of circulating renin, with participation of the liver being predominant.
- Research Organization:
- Osaka City Univ. Medical School, Japan
- OSTI ID:
- 6647280
- Journal Information:
- Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
RENIN
CLEARANCE
EXCRETION
HEPATECTOMY
IODINE 125
KIDNEYS
LABELLED COMPOUNDS
LIVER
NEPHRECTOMY
RATS
TISSUE DISTRIBUTION
TRACER TECHNIQUES
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
GLANDS
HYDROLASES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MEDICINE
NONSPECIFIC PEPTIDASES
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PEPTIDE HYDROLASES
RADIOISOTOPES
RODENTS
SURGERY
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques