Fate of circulating renin in conscious rats

Journal Article · Thu Jan 01 04:00:00 EST 1987 · Am. J. Physiol.; (United States)

OSTI ID:6647280

Kim, S; Iwao, H; Nakamura, N; Ikemoto, F; Yamamoto, K

Highly purified /sup 125/I-labeled rat renal renin (/sup 125/I-renin) was given intravenously to conscious rats to study the fate of circulating renin. Specific antirat renin antiserum was used to identify the labeled renin molecules. In sham-operated rats, the disappearance of /sup 125/I-renin from the plasma showed two exponential components with a half-life of 6.7 +/- 0.4 min for the rapid component and 65.1 +/- 5.7 min for the slow component. The metabolic clearance rate was 11.4 +/- 1.0 ml X min-1 X kg-1. In bilaterally nephrectomized rats, the metabolic clearance rate of /sup 125/I-renin was reduced by 55%, but the half-life of the slow component remained unchanged. Seventy percent hepatectomy caused a 54% decrement in the metabolic clearance and prolonged the half-life of the slow component. Five minutes after injection of /sup 125/I-renin, approximately 59 and 11% of the administered /sup 125/I-renin had accumulated in the liver and the kidneys, respectively, and at later time points the /sup 125/I-renin was highly concentrated in these organs. High-performance liquid chromatographic analysis of the liver and kidney extracts demonstrated that /sup 125/I-renin was catabolized by these organs. Biliary excretion of /sup 125/I-renin was negligible. Urinary excretion of /sup 125/I-renin up to 120 min was approximately 2% of the injected dose. We conclude that both the liver and the kidney are responsible for the clearance of circulating renin, with participation of the liver being predominant.

• Research Organization: Osaka City Univ. Medical School, Japan
• OSTI ID: 6647280
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States); ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CLEARANCE
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
EXCRETION
GLANDS
HEPATECTOMY
HYDROLASES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
LIVER
MAMMALS
MEDICINE
NEPHRECTOMY
NONSPECIFIC PEPTIDASES
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PEPTIDE HYDROLASES
RADIOISOTOPES
RATS
RENIN
RODENTS
SURGERY
TISSUE DISTRIBUTION
TRACER TECHNIQUES
VERTEBRATES