Cellular and subcellular distribution of exogenously administered renal renin in rat liver and kidney

Journal Article · Mon Nov 30 23:00:00 EST 1987 · American Journal of Physiology; (USA)

OSTI ID:7025843

Kim, Shokei; Iwao, Hiroshi; Nakamura, Norifumi; Ikemoto, Fumihiko; Yamamoto, Kenjiro; Mizuhira, Vinci; Yokofujita, Junko ^[1]

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1. Osaka City Univ. Medical School (Japan) Kagawa Medical School (Japan) Tokyo Medical and Dental Univ. (Japan)

Using highly purified {sup 125}I-labeled rat renal renin, the authors have demonstrated that both the liver and kidney are responsible for clearance of renal renin. In the present study, light and electron microscope autoradiography of intravenously administered {sup 125}I-labeled rat renal renin was performed in rat liver and kidney to observe the cellular and subcellular distribution of the renin. Autoradiography was performed. In the liver, silver grains were mainly localized in Kupffer cells and not in hepatocytes. In the kidney, silver grains were evident in the proximal tubule cells. In both liver and kidney cells, silver grains were mainly located over the lysosomes. Gel permeation high-performance liquid chromatography analysis of the liver and kidney extracts indicated two main fractions, including immunoreactive {sup 125}I-renin and the breakdown products (free {sup 125}I and {sup 125}I-tyrosine). In conclusion, circulating renal renin is mainly taken up by Kupffer cells and proximal tubule cells and is subsequently transported to the lysosomes to be degraded. These results, taken together with the previous finding that nonglycosylated submaxillary renin does not distribute in the liver, suggest that the carbohydrate moieties of renal renin are necessary for the recognition by Kupffer cells.

• OSTI ID: 7025843
• Journal Information: American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 253:6; ISSN 0002-9513; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
ELECTRON MICROSCOPY
ENZYMES
GLANDS
HYDROLASES
HYDROXY ACIDS
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
INTRAVENOUS INJECTION
IODINE 125
IODINE ISOTOPES
ISOTOPES
KIDNEYS
LIQUID COLUMN CHROMATOGRAPHY
LIVER
LIVER CELLS
LYSOSOMES
MICROSCOPY
NONSPECIFIC PEPTIDASES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
PEPTIDE HYDROLASES
RADIOISOTOPES
RENIN
SEPARATION PROCESSES
SOMATIC CELLS
SUBCELLULAR DISTRIBUTION
TISSUE DISTRIBUTION
TRANSMISSION ELECTRON MICROSCOPY
TYROSINE