Metabolism of circulating renin by liver and kidney of rats

Journal Article · Thu Jan 01 04:00:00 EST 1987 · J. Cardiovasc. Pharmacol.; (United States)

DOI:https://doi.org/10.1097/00005344-198706107-00016· OSTI ID:6924403

Kim, S; Iwao, H; Nakamura, N; Ikemoto, F; Yamamoto, K; Mizuhira, V; Yokofujita, J

Rat renal renin, highly purified and labeled with /sup 125/I, was intravenously given to conscious rats, to study the fate of the circulating renin. Rat antirenin antiserum was used to identify the labeled renin. The disappearance of (/sup 125/I)-renin from the plasma showed two exponential components and the metabolic clearance rate was 11.4 +/- 1.0 ml/min/kg. Both 70% hepatectomy and bilateral nephrectomy decreased the clearance rate by about 50%. (/sup 125/I)-renin accumulated mainly in the liver and kidney, and high performance liquid chromatography (HPLC) analysis indicated the degradation of (/sup 125/I)-renin by these organs. Biliary excretion of (/sup 125/I)-renin was negligible and urinary excretion accounted for 2% of the injected dose. Light- and electron-microscopic autoradiography indicated that (/sup 125/I)-renin is taken up mainly by Kupffer cells and proximal convoluted tubular cells in the liver and kidney, respectively, and thereafter distributes to the lysosomes. In conclusion, both the liver and kidney are responsible for the clearance of circulating renin.

• Research Organization: Osaka City Univ. Medical School (Japan)
• OSTI ID: 6924403
• Journal Information: J. Cardiovasc. Pharmacol.; (United States), Journal Name: J. Cardiovasc. Pharmacol.; (United States); ISSN JCPCD
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BODY
CHROMATOGRAPHY
CLEARANCE
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
GLANDS
HYDROLASES
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
INTRAVENOUS INJECTION
IODINE 125
IODINE ISOTOPES
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
LIVER
MAMMALS
METABOLISM
NONSPECIFIC PEPTIDASES
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PEPTIDE HYDROLASES
RADIOISOTOPES
RATS
RENIN
RODENTS
SEPARATION PROCESSES
VERTEBRATES