Whole body autoradiographic distribution of exogenously administered renin in mice

Journal Article · Wed Jun 01 04:00:00 EDT 1983 · J. Histochem. Cytochem.; (United States)

DOI:https://doi.org/10.1177/31.6.6341461· OSTI ID:5899616

Iwao, H; Nakamura, N; Ikemoto, F; Yamamoto, K

The distribution of exogenously administered renin was investigated using whole body autoradiography. Purified renin from mouse submaxillary gland (SR) was labeled with radioactive iodine (/sup 125/I). This labeled renin (/sup 125/I-SR) and Na/sup 125/I were administered into the tail vein of male ddY mice, in doses of 10.2 and 16.4 mu Ci/30 g body weight, respectively. Mice were killed by an overdose of ether, and autoradiography was performed on whole body sections. To separate free /sup 125/I liberated from /sup 125/I-SR, sections were treated with perchloric acid. A major accumulation of /sup 125/I-SR, acid-insoluble, was evident in the renal cortex, whereas the hepatic accumulation of /sup 125/I-SR was minor. Radioactivity in the thyroid and submaxillary glands, in the stomach, and in urine was also apparent, but disappeared after acid treatment, except in the thyroid glands. Radioactivity in the brain, intestinal content, spleen, and adrenal glands was nil. These autoradiograms provide the first evidence that exogenously administered renin is mainly distributed in the renal cortex.

• Research Organization: Department of Pharmacology, Osaka City University Medical School, Japan
• OSTI ID: 5899616
• Journal Information: J. Histochem. Cytochem.; (United States), Journal Name: J. Histochem. Cytochem.; (United States) Vol. 31:6; ISSN JHCYA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BODY
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE GLANDS
ENZYMES
GASTROINTESTINAL TRACT
GLANDS
HYDROLASES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
KIDNEYS
LABELLED COMPOUNDS
LIVER
MAMMALS
MICE
NONSPECIFIC PEPTIDASES
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PEPTIDE HYDROLASES
RADIOISOTOPES
RENIN
RODENTS
SALIVARY GLANDS
STOMACH
THYROID
TISSUE DISTRIBUTION
VERTEBRATES
WHOLE-BODY COUNTING