Whole body autoradiographic distribution of exogenously administered renin in mice
The distribution of exogenously administered renin was investigated using whole body autoradiography. Purified renin from mouse submaxillary gland (SR) was labeled with radioactive iodine (/sup 125/I). This labeled renin (/sup 125/I-SR) and Na/sup 125/I were administered into the tail vein of male ddY mice, in doses of 10.2 and 16.4 mu Ci/30 g body weight, respectively. Mice were killed by an overdose of ether, and autoradiography was performed on whole body sections. To separate free /sup 125/I liberated from /sup 125/I-SR, sections were treated with perchloric acid. A major accumulation of /sup 125/I-SR, acid-insoluble, was evident in the renal cortex, whereas the hepatic accumulation of /sup 125/I-SR was minor. Radioactivity in the thyroid and submaxillary glands, in the stomach, and in urine was also apparent, but disappeared after acid treatment, except in the thyroid glands. Radioactivity in the brain, intestinal content, spleen, and adrenal glands was nil. These autoradiograms provide the first evidence that exogenously administered renin is mainly distributed in the renal cortex.
- Research Organization:
- Department of Pharmacology, Osaka City University Medical School, Japan
- OSTI ID:
- 5899616
- Journal Information:
- J. Histochem. Cytochem.; (United States), Vol. 31:6
- Country of Publication:
- United States
- Language:
- English
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IODINE 125
AUTORADIOGRAPHY
RENIN
TISSUE DISTRIBUTION
KIDNEYS
LABELLED COMPOUNDS
LIVER
MICE
SALIVARY GLANDS
STOMACH
THYROID
WHOLE-BODY COUNTING
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE GLANDS
ENZYMES
GASTROINTESTINAL TRACT
GLANDS
HYDROLASES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
MAMMALS
NONSPECIFIC PEPTIDASES
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PEPTIDE HYDROLASES
RADIOISOTOPES
RODENTS
VERTEBRATES
550501* - Metabolism- Tracer Techniques