Fate of recombinant human renin administered exogenously to anesthetized monkeys

Journal Article · Thu Sep 01 04:00:00 EDT 1988 · Hypertension (Dallas); (United States)

DOI:https://doi.org/10.1161/01.HYP.12.3.317· OSTI ID:6558614

Hiruma, M; Kim, S; Ikemoto, F; Murakami, K; Yamamoto, K

Highly purified recombinant human renin (rh-renin), synthesized by Chinese hamster ovary cells, was labeled with iodine-125 and was given intravenously to pentobarbital-anesthetized common marmosets (Callithrix jacchus) to study the fate of the circulating renin. Specific anti-rh-renin antiserum was used to identify the 125I-rh-renin. Plasma disappearance of the exogenously administered 125I-rh-renin in marmosets (n = 6) showed two exponential components, with a half-life of 12.1 +/- 1.9 minutes for the rapid component and 120.3 +/- 16.4 minutes for the slow component. The metabolic clearance rate was 1.17 +/- 0.26 ml/min/kg. Thirty minutes after the injection of 125I-rh-renin, 43.1 +/- 0.9 and 3.5 +/- 0.5% of the injected dose had distributed to the liver and the kidneys, respectively. With time, the accumulated 125I-rh-renin in the liver and kidneys decreased. The accumulation of 125I-rh-renin was less than 1% of the dose injected in other organs such as lungs, heart, spleen, adrenal glands, testes, and ovaries. Analysis of liver and kidney extracts by high performance liquid chromatography at 30 and 120 minutes indicated that immunoreactive 125I-rh-renin decreased with time and was accompanied by an increase in nonimmunoreactive degradation products of a low molecular weight. The incubation of 125I-rh-renin with monkey or human plasma at 37 degrees C did not degrade the labeled renin. Therefore, rh-renin was rapidly cleared from the circulation by the liver and the kidney.

• Research Organization: Osaka City Univ., Medical School (Japan)
• OSTI ID: 6558614
• Journal Information: Hypertension (Dallas); (United States), Journal Name: Hypertension (Dallas); (United States) Vol. 12:3; ISSN HPRTD
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANESTHESIA
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL HALF-LIFE
BLOOD PRESSURE
CHROMATOGRAPHY
CLEARANCE
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
HYDROLASES
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
INTRAVENOUS INJECTION
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
METABOLISM
MONKEYS
NONSPECIFIC PEPTIDASES
NUCLEI
ODD-EVEN NUCLEI
PEPTIDE HYDROLASES
PRIMATES
RADIOISOTOPES
RENIN
SEPARATION PROCESSES
TISSUE DISTRIBUTION
TRACER TECHNIQUES
VERTEBRATES