Quantitation of ultraviolet radiation-induced cyclobutyl pyrimidine dimers in DNA by video and photographic densitometry

Journal Article · Tue May 01 00:00:00 EDT 1990 · Analytical Biochemistry; (USA)

DOI:https://doi.org/10.1016/0003-2697(90)90070-P· OSTI ID:6640226

Freeman, S E; Thompson, B D ^[1]

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1. Lovelace Medical Foundation, Albuquerque, NM (USA)

We have compared video and photographic methods for calculating the number of ultraviolet radiation (uv)-induced pyrimidine dimers in DNA from the bacteriophage T7 exposed to uv (0 to 800 J/m2) from an FS40 sunlamp. DNA was incubated with a pyrimidine dimer-specific Micrococcus luteus uv endonuclease, subjected to alkaline agarose gel electrophoresis, neutralized, and stained with ethidium bromide, and the DNA fluorescence was recorded either with a video camera or on photographic film. The slopes of the dose-response curves for the number of uv-endonuclease-sensitive sites per 10(3) bases (pyrimidine dimers) was 1.2 (+/- 0.1) X 10(-4) uv-endonuclease-sensitive sites per J/m2 for the video analysis and 1.3 (+/- 0.04) X 10(-4) uv-endonuclease-sensitive sites per J/m2 for the photographic analysis. Results for pyrimidine dimer determination by either method were statistically comparable.

• OSTI ID: 6640226
• Journal Information: Analytical Biochemistry; (USA), Vol. 186:2; ISSN 0003-2697
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIOPHAGES
GENETIC RADIATION EFFECTS
PYRIMIDINE DIMERS
RADIOINDUCTION
DNA
DOSE-RESPONSE RELATIONSHIPS
ELECTROPHORESIS
ENDONUCLEASES
FLUORESCENCE
MICROCOCCUS LUTEUS
ULTRAVIOLET RADIATION
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
DNA-ASE
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERASES
GENETIC EFFECTS
HYDROLASES
LUMINESCENCE
MICROCOCCUS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PARASITES
PHOSPHODIESTERASES
RADIATION EFFECTS
RADIATIONS
VIRUSES
560130* - Radiation Effects on Microorganisms