Photoreactivation of ultraviolet radiation-induced pyrimidine dimers in neonatal BALB/c mouse skin
The numbers of ultraviolet light (uv)-induced pyrimidine dimers in the DNA of neonatal BALB/c mouse skin were measured by assessing the sensitivity of the DNA to Micrococcus luteus uv endonuclease. Irradiation of neonatal BALB/c mice with FS40 sunlamps caused a dose-dependent induction of endonuclease-sensitive sites (pyrimidine dimers) in DNA extracted from back skin. Exposure of these uv-irradiated neonatal mice to photoreactivating (PR) light (cool white fluorescent lamp and incandescent lamp) caused a reduction in the number of pyrimidine dimers in the DNA, as revealed by a shift in low-molecular-weight DNA to high-molecular-weight DNA. In contrast, DNA profiles of the skin of either uv-irradiated mice or uv-irradiated mice kept in the dark for the same duration as those exposed to PR light did not show a loss of uv-induced endonuclease-sensitive sites. Furthermore, reversing the order of treatment, i.e., administering PR light first and then uv, did not produce a reduction in pyrimidine dimers. These results demonstrate that PR or uv-induced pyrimidine dimers occurs in neonatal BALB/c mouse skin. The optimal wavelength range for in vivo PR appears to be in the visible region of the spectrum (greater than 400 nm). Although dimer formation could be detected in both dermis and epidermis, PR occurred only in the dermis. Furthermore, the PR phenomenon could not be detected in the skin of adult mice from the same inbred strain.
- OSTI ID:
- 5703465
- Journal Information:
- Cancer Res.; (United States), Vol. 41:5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
DNA
ULTRASTRUCTURAL CHANGES
SKIN
RADIOSENSITIVITY
AGE DEPENDENCE
BIOCHEMISTRY
DIMERS
DOSE-RESPONSE RELATIONSHIPS
MICE
MICROCOCCUS LUTEUS
NEONATES
NUCLEASES
PHOTOREACTIVATION
PYRIMIDINES
ULTRAVIOLET RADIATION
VISIBLE RADIATION
ANIMALS
AZINES
BACTERIA
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CHEMISTRY
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERASES
HETEROCYCLIC COMPOUNDS
HYDROLASES
MAMMALS
MICROCOCCUS
MICROORGANISMS
MORPHOLOGICAL CHANGES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHOSPHODIESTERASES
RADIATIONS
RECOVERY
REPAIR
RODENTS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals
560114 - Radiation Effects on Biochemicals- In Animals- (-1987)
550200 - Biochemistry