Excision repair of UV-induced pyrimidine dimers in human skin in vivo

Journal Article · Tue Sep 01 00:00:00 EDT 1981 · J. Invest. Dermatol.; (United States)

DOI:https://doi.org/10.1111/1523-1747.ep12482484· OSTI ID:5402367

D'Ambrosio, S M; Slazinski, L; Whetstone, J W; Lowney, E

The induction and loss of pyrimidine dimers in human skin in vivo was determined using UV endonuclease, alkaline sucrose sedimentations, and the fluorescent detection of nonradiolabeled DNA. The number of dimers induced following exposure of the skin to radiation emitted from a Burdick UV-800 sunlamp was quantitated by reacting the extracted DNA with Micrococcus luteus endonuclease specific for pyrimidine dimers. Exposure to 15 and 30 seconds of radiation emitted from this lamp produced the formation of 12.8 and 23.6 dimers per 10(8) daltons DNA, respectively. Approximately 50% of the dimers induced were lost 58 min after irradiation. Only a small percentage (less than 10) remained 24 hr postirradiation. These data partially characterize the process by which pyrimidine dimers are excised from human skin DNA in vivo.

• OSTI ID: 5402367
• Journal Information: J. Invest. Dermatol.; (United States), Journal Name: J. Invest. Dermatol.; (United States) Vol. 77:3; ISSN JIDEA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560115* -- Radiation Effects on Biochemicals-- In Man-- (-1987)
560151 -- Radiation Effects on Animals-- Man
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AZINES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
CHEMICAL RADIATION EFFECTS
CHEMISTRY
DIMERS
DNA
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERASES
HETEROCYCLIC COMPOUNDS
HYDROLASES
IN VIVO
NUCLEASES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHOSPHODIESTERASES
PYRIMIDINES
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SKIN
ULTRAVIOLET RADIATION