Excision repair of UV-induced pyrimidine dimers in human skin in vivo
The induction and loss of pyrimidine dimers in human skin in vivo was determined using UV endonuclease, alkaline sucrose sedimentations, and the fluorescent detection of nonradiolabeled DNA. The number of dimers induced following exposure of the skin to radiation emitted from a Burdick UV-800 sunlamp was quantitated by reacting the extracted DNA with Micrococcus luteus endonuclease specific for pyrimidine dimers. Exposure to 15 and 30 seconds of radiation emitted from this lamp produced the formation of 12.8 and 23.6 dimers per 10(8) daltons DNA, respectively. Approximately 50% of the dimers induced were lost 58 min after irradiation. Only a small percentage (less than 10) remained 24 hr postirradiation. These data partially characterize the process by which pyrimidine dimers are excised from human skin DNA in vivo.
- OSTI ID:
- 5402367
- Journal Information:
- J. Invest. Dermatol.; (United States), Vol. 77:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
DNA
BIOLOGICAL REPAIR
CHEMICAL RADIATION EFFECTS
DIMERS
IN VIVO
NUCLEASES
PYRIMIDINES
SKIN
ULTRAVIOLET RADIATION
AZINES
BIOLOGICAL RECOVERY
BODY
CHEMISTRY
ELECTROMAGNETIC RADIATION
ENZYMES
ESTERASES
HETEROCYCLIC COMPOUNDS
HYDROLASES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHOSPHODIESTERASES
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
560115* - Radiation Effects on Biochemicals- In Man- (-1987)
560151 - Radiation Effects on Animals- Man
550200 - Biochemistry