Pyrimidine dimer formation and repair in human skin

Journal Article · Mon Sep 01 00:00:00 EDT 1980 · Cancer Res.; (United States)

OSTI ID:7029913

Sutherland, B M; Harber, L C; Kochevar, I E

Cyclobutyl pyrimidine dimers have been detected in the DNA of human skin following in vivo irradiation with suberythermal doses of ultraviolet (UV) radiation from FS-20 sun lamp fluorescent tubes. Dimers were assayed by treatment of extracted DNA with Micrococus luteus UV-specific endonuclease, alkaline agarose electrophoresis, and ethidum bromide staining. This technique, in contrast to conventional dimer assays, can be used with nonradioactive DNA and is optimal at low UV light doses. These data suggest that some dimer disappearance by excision repair occurs within 20 min of UV irradiation and that photoreactivation of dimers can make a contribution to the total repair process.

• Research Organization: Brookhaven National Lab., Upton, NY
• OSTI ID: 7029913
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 40; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AZINES
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
DIMERS
DNA
ELECTROMAGNETIC RADIATION
ELECTROPHORESIS
ENZYMES
ERYTHEMA
ESTERASES
HETEROCYCLIC COMPOUNDS
HISTOLOGICAL TECHNIQUES
HYDROLASES
MAMMALS
MAN
MICROCOCCUS
MICROCOCCUS LUTEUS
MICROORGANISMS
NUCLEASES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHOSPHODIESTERASES
PHOTOREACTIVATION
PRIMATES
PYRIMIDINES
RADIATIONS
RADIOINDUCTION
RECOVERY
REPAIR
SKIN
SYMPTOMS
ULTRAVIOLET RADIATION
VERTEBRATES