Induction and persistence of pyrimidine dimers in the epidermal DNA of two strains of hairless mice

Journal Article · Thu Sep 01 04:00:00 EDT 1977 · Cancer Res.; (United States)

OSTI ID:5798860

Ley, R D; Sedita, B A; Grube, D D; Fry, R J.M.

The ultraviolet-light induction of DNA damage has been measured in the epidermis of hairless mice with the use of damage-specific endonucleases from Micrococcus luteus. The rates of induction of endonuclease-sensitive sites in HRS/J/Anl and SKH:hairless-1 mice were 6.1 +- 0.5 x 10/sup -11/ and 6.5 +- 0.8 x 10/sup -11//dalton/J/sq m from a FS40 fluorescent sun lamp (280 to 400 nm), respectively. Enzymatic photoreactivation with yeast photoreactivating enzyme showed that approximately 80% of the endonuclease-sensitive sites were cyclobutyl pyrimidine dimers. In both strains of mice the pyrimidine dimers remained in high-molecular-weight DNA for 24 hr after irradiation. These data show that mouse epithelial cells in vivo have little or no capacity for the excision repair of pyrimidine dimers.

• Research Organization: Argonne National Lab., IL
• OSTI ID: 5798860
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 37; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
560400 -- Other Environmental Pollutant Effects
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AZINES
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BODY
DIMERS
DNA
ELECTROMAGNETIC RADIATION
ENZYMES
EPIDERMIS
EPITHELIUM
FUNGI
HETEROCYCLIC COMPOUNDS
MAMMALS
MICE
MICROCOCCUS
MICROCOCCUS LUTEUS
MICROORGANISMS
NUCLEASES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHOSPHOTRANSFERASES
PHOTOREACTIVATION
PLANTS
PYRIMIDINES
RADIATIONS
RECOVERY
REPAIR
RODENTS
SKIN
TISSUES
TRANSFERASES
ULTRAVIOLET RADIATION
VERTEBRATES
YEASTS