Induction and persistence of pyrimidine dimers in the epidermal DNA of two strains of hairless mice

Journal Article · Thu Sep 01 00:00:00 EDT 1977 · Cancer Res.; (United States)

OSTI ID:5798860

Ley, R D; Sedita, B A; Grube, D D; Fry, R J.M.

The ultraviolet-light induction of DNA damage has been measured in the epidermis of hairless mice with the use of damage-specific endonucleases from Micrococcus luteus. The rates of induction of endonuclease-sensitive sites in HRS/J/Anl and SKH:hairless-1 mice were 6.1 +- 0.5 x 10/sup -11/ and 6.5 +- 0.8 x 10/sup -11//dalton/J/sq m from a FS40 fluorescent sun lamp (280 to 400 nm), respectively. Enzymatic photoreactivation with yeast photoreactivating enzyme showed that approximately 80% of the endonuclease-sensitive sites were cyclobutyl pyrimidine dimers. In both strains of mice the pyrimidine dimers remained in high-molecular-weight DNA for 24 hr after irradiation. These data show that mouse epithelial cells in vivo have little or no capacity for the excision repair of pyrimidine dimers.

• Research Organization: Argonne National Lab., IL
• OSTI ID: 5798860
• Journal Information: Cancer Res.; (United States), Vol. 37
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
DNA
BIOLOGICAL REPAIR
ULTRAVIOLET RADIATION
BIOLOGICAL EFFECTS
DIMERS
EPIDERMIS
MICE
MICROCOCCUS LUTEUS
NUCLEASES
PHOTOREACTIVATION
PYRIMIDINES
YEASTS
ANIMALS
AZINES
BACTERIA
BIOLOGICAL RECOVERY
BODY
ELECTROMAGNETIC RADIATION
ENZYMES
EPITHELIUM
FUNGI
HETEROCYCLIC COMPOUNDS
MAMMALS
MICROCOCCUS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHOSPHOTRANSFERASES
PLANTS
RADIATIONS
RECOVERY
REPAIR
RODENTS
SKIN
TISSUES
TRANSFERASES
VERTEBRATES
550200* - Biochemistry
560400 - Other Environmental Pollutant Effects