Induction of pyrimidine dimers in epidermal DNA of hairless mice by UVB: an action spectrum

Journal Article · Mon Feb 28 23:00:00 EST 1983 · J. Invest. Dermatol.; (United States)

DOI:https://doi.org/10.1111/1523-1747.ep12534010· OSTI ID:5816038

Ley, R D; Peak, M J; Lyon, L L

An action spectrum for the induction of pyrimidine dimers in the epidermis of hairless mice was determined between 288 and 307 nm. The presence of pyrimidine dimers in tritium-labeled DNA extracted from exposed SKH:hairless-1 mouse skin was determined using dimer-specific nucleases from Micrococcus luteus in conjunction with sedimentation of the irradiated DNA in alkaline sucrose gradients. The rate of induction of pyrimidine dimers was maximal at 293 nm. These values were used to propose a UVB transmission curve for mouse epidermis.

• Research Organization: Biology Division, Oak Ridge National Laboratory, Tennessee
• OSTI ID: 5816038
• Journal Information: J. Invest. Dermatol.; (United States), Journal Name: J. Invest. Dermatol.; (United States) Vol. 80:3; ISSN JIDEA
• Country of Publication: United States
• Language: English

Related Subjects

560114* -- Radiation Effects on Biochemicals-- In Animals-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AZINES
BODY
CHEMICAL RADIATION EFFECTS
CHEMISTRY
DIMERS
DNA
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
LABELLED COMPOUNDS
MAMMALS
MICE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RODENTS
SKIN
SPECTRA
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
ULTRAVIOLET SPECTRA
VERTEBRATES