Evaluations of cellular proliferation and chromosome breakages after in vitro exposure of human lymphocytes to calcium or zinc DTPA
The analysis of mitotic indices (MI) and chromosome breakages in metaphases of 50-hr lymphocyte cultures exposed to the calcium or zinc chelates of diethylenetriamine pentaacetic acid (DTPA) demonstrated: (1) an 80% reduction in MI in cultures from three women but no reduction in those from two men after in vitro exposure to CaDTPA in concentrations as low as 10..mu..g/ml culture medium, and complete suppression of mitoses in cultures from men and women after exposure to 40 ..mu..g/ml CaDTPA; (2) minor suppression in MI in cultures from women and none in those from men after exposure to 40 or 80 ..mu..g/ml ZnDTPA; (3) no ring or dicentric chromosomes in 1700 metaphases from DTPA-treated cultures. Likewise, the authors observed no differences in the frequency or distributions of rings and dicentrics in lymphocyte cultures from two persons after in vitro exposure to 250-R /sup 60/Co ..gamma.. radiation in the presence or absence of 10 ..mu..g/ml CaDTPA or 10 or 80 ..mu..g/ml ZnDTPA.
- Research Organization:
- Oak Ridge Associated Universities, TN
- DOE Contract Number:
- AC05-76OR00033
- OSTI ID:
- 6599825
- Journal Information:
- Radiat. Res.; (United States), Vol. 99:1
- Country of Publication:
- United States
- Language:
- English
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CHROMOSOME BREAKAGE
RADIOINDUCTION
DTPA
RADIOSENSITIVITY EFFECTS
LYMPHOCYTES
BIOLOGICAL RADIATION EFFECTS
CELL PROLIFERATION
CALCIUM COMPLEXES
COBALT 60
GAMMA RADIATION
IN VITRO
MITOTIC INDEX
ZINC COMPLEXES
ALKALINE EARTH METAL COMPLEXES
AMINO ACIDS
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CHELATING AGENTS
CHROMOSOMAL ABERRATIONS
COBALT ISOTOPES
COMPLEXES
CONNECTIVE TISSUE CELLS
DRUGS
ELECTROMAGNETIC RADIATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LEUKOCYTES
MATERIALS
MINUTES LIVING RADIOISOTOPES
MUTATIONS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
SOMATIC CELLS
YEARS LIVING RADIOISOTOPES
560151* - Radiation Effects on Animals- Man
560161 - Radionuclide Effects
Kinetics
& Toxicology- Man