Evaluations of cellular proliferation and chromosome breakages after in vitro exposure of human lymphocytes to calcium or zinc DTPA

Journal Article · Sun Jul 01 04:00:00 EDT 1984 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576451· OSTI ID:6599825

Littlefield, L G; Joiner, E E; Colyer, S P; DuFrain, R J; Washburn, L C

The analysis of mitotic indices (MI) and chromosome breakages in metaphases of 50-hr lymphocyte cultures exposed to the calcium or zinc chelates of diethylenetriamine pentaacetic acid (DTPA) demonstrated: (1) an 80% reduction in MI in cultures from three women but no reduction in those from two men after in vitro exposure to CaDTPA in concentrations as low as 10..mu..g/ml culture medium, and complete suppression of mitoses in cultures from men and women after exposure to 40 ..mu..g/ml CaDTPA; (2) minor suppression in MI in cultures from women and none in those from men after exposure to 40 or 80 ..mu..g/ml ZnDTPA; (3) no ring or dicentric chromosomes in 1700 metaphases from DTPA-treated cultures. Likewise, the authors observed no differences in the frequency or distributions of rings and dicentrics in lymphocyte cultures from two persons after in vitro exposure to 250-R /sup 60/Co ..gamma.. radiation in the presence or absence of 10 ..mu..g/ml CaDTPA or 10 or 80 ..mu..g/ml ZnDTPA.

• Research Organization: Oak Ridge Associated Universities, TN
• DOE Contract Number: AC05-76OR00033
• OSTI ID: 6599825
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 99:1; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560151* -- Radiation Effects on Animals-- Man
560161 -- Radionuclide Effects
Kinetics
& Toxicology-- Man
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH METAL COMPLEXES
AMINO ACIDS
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CALCIUM COMPLEXES
CARBOXYLIC ACIDS
CELL PROLIFERATION
CHELATING AGENTS
CHROMOSOMAL ABERRATIONS
CHROMOSOME BREAKAGE
COBALT 60
COBALT ISOTOPES
COMPLEXES
CONNECTIVE TISSUE CELLS
DRUGS
DTPA
ELECTROMAGNETIC RADIATION
GAMMA RADIATION
IN VITRO
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MINUTES LIVING RADIOISOTOPES
MITOTIC INDEX
MUTATIONS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
RADIOSENSITIVITY EFFECTS
SOMATIC CELLS
YEARS LIVING RADIOISOTOPES
ZINC COMPLEXES