The persistence of lymphocytes with dicentric chromosomes following whole-body X irradiation of mice

Journal Article · Mon Oct 01 04:00:00 EDT 1990 · Radiation Research; (USA)

DOI:https://doi.org/10.2307/3577689· OSTI ID:6067457

Kligerman, A D; Halperin, E C; Erexson, G L; Honore, G ^[1]

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1. Environmental Protection Agency, Research Triangle Park, NC (USA)

Thirty-six male C57B1/6 mice were X-irradiated whole body with 3 Gy to generate lymphocytes with dicentric chromosomes to study the persistence of these lymphocytes in the spleen and peripheral blood to estimate the life span of mature B- and T-cells. Peripheral blood and spleen were removed from groups of four mice immediately after radiation exposure and on Days 1, 3, 7, 14, 28, 56, and 112 thereafter. Peripheral blood lymphocytes were cultured with phytohemagglutinin to stimulate T-cell division, and splenic lymphocytes were cultured with either lipopolysaccharide or phytohemagglutinin to stimulate B- or T-cell division, respectively. The initial frequencies of dicentric chromosomes with accompanying fragments observed in splenic T-cells (0.44), splenic B-cells (0.43), and peripheral blood lymphocyte cultures (0.48) initiated on Day 0 were not significantly different. For both splenic and peripheral blood T-lymphocytes, the frequency of cells containing dicentric chromosomes declined in an exponential manner following irradiation, with a 50% reduction in frequency occurring 14 days after exposure. In contrast, the frequency of B-cells containing dicentric chromosomes remained stable through Day 7 but then declined precipitously between Day 7 and Day 14 and remained relatively stable, although slightly above baseline, through Day 112 post-exposure. For both B- and T-cells, less than 5% of the cells contained a dicentric chromosome with accompanying fragments at Day 112. These data indicate that B- and T-lymphocytes with dicentric chromosomes show different decay kinetics and suggest that they may possess different life spans.

• OSTI ID: 6067457
• Journal Information: Radiation Research; (USA), Journal Name: Radiation Research; (USA) Vol. 124:1; ISSN 0033-7587; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGGLUTININS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOHYDRATES
CELL CYCLE
CELL DIVISION
CHROMOSOMAL ABERRATIONS
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HEMAGGLUTININS
IONIZING RADIATIONS
IRRADIATION
LEUKOCYTES
LIFE SPAN
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
MITOGENS
MUCOPROTEINS
MUTATION FREQUENCY
MUTATIONS
ORGANIC COMPOUNDS
ORGANS
PHYTOHEMAGGLUTININ
POLYSACCHARIDES
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RODENTS
SACCHARIDES
SOMATIC CELLS
SPLEEN
VERTEBRATES
WHOLE-BODY IRRADIATION
X RADIATION