Computer-assisted morphometric analysis of radiation injury in murine lymphocytes
Groups of mice were killed 1, 3, 7 and 15 days after whole body exposure to 0, 5, 50 or 500 rads of irradiation. Lymphocytes were harvested from the peripheral blood and spleen for total and differential leukocyte counts, for determination of the absolute numbers of thymic-dependent (T) and bone marrow-dependent (B) cells and the in vitro responsiveness to phytohemagglutinin (PHA), concanavalin A (Con A) and lipopolysaccharide (LPS) mitogens and for computer-assisted morphometric analysis (CAMA) of Feulgen-stained nuclei. Alterations in functional activity and morphometric composition showed the same trends with respect to radiation injury, with maximum effects observed three days after exposure. The spleen contained a greater proportion of altered cells than did the peripheral blood. Comparison of the results with similar data obtained from lymphocytes irradiated in vitro showed that the same micromorphometric descriptors serve to distinguish irradiated from control cells in both cases.
- Research Organization:
- Department of Microbiology, University of Arizona, Tucson
- OSTI ID:
- 6186848
- Journal Information:
- Anal. Quant. Cytol.; (United States), Journal Name: Anal. Quant. Cytol.; (United States) Vol. 4:3; ISSN AQCYD
- Country of Publication:
- United States
- Language:
- English
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560152* -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
DOSES
EXTERNAL IRRADIATION
IRRADIATION
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
MORPHOLOGICAL CHANGES
PATHOLOGY
RADIATION DOSES
RADIATION EFFECTS
RODENTS
SOMATIC CELLS
TIME DEPENDENCE
VERTEBRATES
WHOLE-BODY IRRADIATION