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Persistence of lymphocytes with dicentric chromosomes following whole-body x irradiation of mice

Technical Report ·
OSTI ID:5732833
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Thirty-six male C57B1/6 mice were whole-body x-irradiated with 3 Gy to generate lymphocytes with dicentric chromosomes to study the persistence of these lymphocytes in the spleen and peripheral blood to estimate the lifespan of mature B- and T-cells. Blood and spleen were removed from groups of four mice immediately after radiation exposure and on days 1, 3, 7, 14, 28, 56, and 12, thereafter. The initial frequencies of dicentric chromosomes with accompanying fragments observed in splenic T-cells (0.44), splenic B-cells (0.43), and peripheral blood lymphocyte cultures (0.48) initiated on day 0 were not significantly different. For both splenic and peripheral blood-T-lymphocytes, the frequency of cells containing dicentric chromosomes declined in an exponential manner following irradiation, with a 50% reduction in frequency occurring 14 days after exposure. In contrast, the frequency of B-cells containing dicentric chromosomes remained stable through day 7 but then declined precipitously between day 7 and 14 and remained relatively stable, although slightly above baseline, through day 112 post-exposure.
Research Organization:
Environmental Protection Agency, Research Triangle Park, NC (USA). Health Effects Research Lab.
OSTI ID:
5732833
Report Number(s):
PB-91-171868/XAB; EPA--600/J-90/431
Country of Publication:
United States
Language:
English

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Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMOSOMAL ABERRATIONS
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
EXTERNAL IRRADIATION
IONIZING RADIATIONS
IRRADIATION
KINETICS
LEUKOCYTES
LIFE SPAN
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
MUTATIONS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RODENTS
SOMATIC CELLS
VERTEBRATES
WHOLE-BODY IRRADIATION
X RADIATION