Sister chromatid exchange distributions in rabbit lymphocytes treated with streptonigrin
Journal Article
·
· Environ. Manage.; (United States)
Streptonigrin (NSC-45383), a direct-acting clastogen which induces SCEs in vivo and chromosome aberrations both in vivo and in vitro, was evaluated for SCE induction in both G/sub 0/ and stimulated rabbit lymphocytes. Determinations were made for 16 cultures from seven female rabbits. These included controls as well as cells exposed to 90 ..mu..g/kg in vivo, cells pulse-treated with 50 ng/ml in vitro, and a culture continously exposed to 5 ng/ml in vitro. For all cultures the SCE/cell frequency was determined from 20 complete (44 chromosome) metaphases and, in selected cultures, SCEs on individual chromosomes (880 per culture from 20 cells) were enumerated to determine SCE/chromosome frequency and the chromosomal distribution of SCEs. Analysis of variance and least significant difference tests of the ..sqrt..x transformed SCE/cell data show that cells exposed to streptonigrin while dividing have significantly higher frequencies (over double the control 5.3 SCE/cell value) whereas treated G/sub 0/ cells were not significantly different from the controls. Dispersion analysis of both SCE/cell and SCE/chromosome data confirms the adequacy of the Poisson distribution for spontaneous or baseline but not streptonigrin-induced SCEs.
- Research Organization:
- Oak Ridge Associated Universities, TN
- DOE Contract Number:
- AC05-76OR00033
- OSTI ID:
- 5014937
- Journal Information:
- Environ. Manage.; (United States), Journal Name: Environ. Manage.; (United States) Vol. 5:6; ISSN EMNGD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550400 -- Genetics
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CYCLE
CHROMOSOMAL ABERRATIONS
CONNECTIVE TISSUE CELLS
DRUGS
GENETIC EFFECTS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MUTATIONS
RABBITS
SISTER CHROMATID EXCHANGES
SOMATIC CELLS
VERTEBRATES
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CYCLE
CHROMOSOMAL ABERRATIONS
CONNECTIVE TISSUE CELLS
DRUGS
GENETIC EFFECTS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MUTATIONS
RABBITS
SISTER CHROMATID EXCHANGES
SOMATIC CELLS
VERTEBRATES