Effect of cholic acid on the colonic sister chromatid exchange frequency induced by 1,2-dimethylhydrazine in C57BL/6 mice
- Ludwig Institute for Cancer Research, Toronto, Ontario (Canada)
Induction of sister chromatid exchangers (SCE) has been used as evidence of genotoxicity of test compounds administered both in vitro and in vivo. The authors have previously reported their efforts to measure SCE frequencies in colonic tissue in vivo. While the method described did produce chromosome preparations suitable for SCE analysis, the yield of differentiated well-spread metaphase chromosomes was sometimes relatively low, making scoring more time consuming than for determination of SCE frequencies in most cell types. They speculated that if the mitotic index of the tissue could be increased, the yield of metaphases suitable for scoring SCEs would also be increased, reducing the time required to obtain results. Inclusion of cholic acid in the diet of experimental animals is known to produce marked increases in the mitotic activity of colonic tissue, thus providing a possible means by which to effect the desired improvements. The authors report here on the effect of dietary cholic acid on the SCE frequencies induced in murine colonic tissue by the colon carcinogen 1,2-dimethylhydrazine (DMH).
- OSTI ID:
- 5848313
- Journal Information:
- Environmental and Molecular Mutagenesis; (USA), Journal Name: Environmental and Molecular Mutagenesis; (USA) Vol. 17:1; ISSN EMMUE; ISSN 0893-6692
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BILE ACIDS
BIOLOGICAL EFFECTS
CARBOXYLIC ACIDS
CHOLIC ACID
CHROMOSOMAL ABERRATIONS
DOSE-RESPONSE RELATIONSHIPS
GENETIC EFFECTS
HYDRAZINE
HYDROXY COMPOUNDS
MAMMALS
MICE
MITOTIC INDEX
MUTATION FREQUENCY
MUTATIONS
NITROGEN COMPOUNDS
ORGANIC ACIDS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
RODENTS
SISTER CHROMATID EXCHANGES
STEROIDS
STEROLS
VERTEBRATES