Effect of diesel exhaust emissions, particulates, and extract on sister chromatid exchange in transplacentally exposed fetal hamster liver

Journal Article · Fri Jan 01 04:00:00 EST 1982 · Environ. Mutagen.; (United States)

DOI:https://doi.org/10.1002/em.2860040303· OSTI ID:6712144

Pereira, M A ^[1]; McMillan, L; Kaur, P; Gulati, D K; Sabharwal, P S

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1. U.S. Environmental Protection Agency, Cincinnati, OH

The genotoxic activity of diesel exhaust emissions, particulate matter, and an organic extract of the particulate matter was evaluated in transplacentally exposed Syrian hamster fetal liver cells. The frequency of sister chromatid exchange (SCE) was determined on day 13 of gestation. The extract of diesel particulate matter caused a dose-dependent increase in the frequency of SCE with a doubling in the incidence above 320mg/kg. The diesel particulate matter and diesel exhaust emissions did not alter the frequency of SCE. The extract and particulate matter did cause a dose-dependent decrease in the mitotic activity of the fetal liver. The in utero SCE analysis was demonstrated to be a sensitive assay for determinations of the genotoxic activity of a complex mixture in transplacentally exposed fetuses.

• OSTI ID: 6712144
• Journal Information: Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 4:3; ISSN ENMUD
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOASSAY
BIOLOGICAL EFFECTS
BODY
CHROMOSOMAL ABERRATIONS
DIESEL ENGINES
DIESEL FUELS
DIGESTIVE SYSTEM
DOSE-RESPONSE RELATIONSHIPS
ENGINES
EXHAUST GASES
FETUSES
FLUIDS
GASEOUS WASTES
GASES
GLANDS
HAMSTERS
HEAT ENGINES
INTERNAL COMBUSTION ENGINES
LIVER
MAMMALS
MUTATIONS
ORGANS
PARTICLES
PARTICULATES
PETROLEUM PRODUCTS
RODENTS
SISTER CHROMATID EXCHANGES
VERTEBRATES
WASTES