Combined capillary electrophoresis and electrospray ionization mass spectrometry
The development of new capillary electrophoresis (CE) methods provides a basis for the efficient manipulation and separation of subpicomole quantities of polypeptides and proteins. Recent advances in microscale methods, such as the demonstration of the tryptic digestion of low picomole quantities of proteins using the immobilized enzyme in small diameter packed reactor column, provide the basis for such further developments. The use of capillary (free solution) zone electrophoresis (CZE) for separation of proteins, and recent demonstrations of restriction mapping of large deoxyribonucleotides, has propelled potential CE applications into the realm of conventional electrophoresis, while adding the attributes of speed, relatively simple on-line detection, automation, and reduced sample requirements (10{sup {minus}17} {minus} 10 {sup {minus}13} mole). A literal explosion of ancillary methods for sample manipulation, derivatization, and detection as well as new methods of obtaining separation selectivity are being reported. Additionally, other CE formats are attracting increased interest, with the aim of exploiting the unique features of capillary isotachophoresis (CITP), capillary isoelectric focusing (CIEF), capillary electrokinetic chromatograpgy (CEC), and most recently, capillary polyacrylamide gel electrophoresis (CGE). As a result, there are concomitant and increasing demands upon detector sensitivity and information density.
- Research Organization:
- Pacific Northwest Lab., Richland, WA (USA)
- Sponsoring Organization:
- DOE/ER; NSF
- DOE Contract Number:
- AC06-76RL01830
- OSTI ID:
- 6594739
- Report Number(s):
- PNL-SA-18081; CONF-9007162--1; ON: DE90016535
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
400102 -- Chemical & Spectral Procedures
400105* -- Separation Procedures
550200 -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ELECTROPHORESIS
IONIZATION
MASS SPECTROSCOPY
ORGANIC COMPOUNDS
PEPTIDES
POLYMERS
POLYPEPTIDES
PROTEINS
SEPARATION PROCESSES
SPECTROSCOPY