Capillary isotachophoresis mass spectrometry

Journal Article · Tue Jan 31 23:00:00 EST 1989 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00178a008· OSTI ID:6190260

Udseth, H R; Loo, J A; Smith, R D

The on-line combination of capillary isotachophoresis (CITP) with mass spectrometry is demonstrated for the first time. The CITP/MS interface is based upon electrospray ionization and is identical with that developed previously for capillary zone electrophoresis (CZE)/MS. Separations were conducted in untreated 100 ..mu..m i.d. fused silica capillaries having lengths of 0.6-2.5 m, at voltages up to 35 kV. The method involves elution of the leading electrolyte to the electrospray source followed by a sequence of separated analyte bands (if sufficient time is provided for development) and, finally, the trailing electrolyte. The CITP/MS was demonstrated to allow very high resolution separations of quaternary phosphonium ions and other ionic substances having very small differences in electrophoretic mobilities. Nearly ideal band shapes are obtained in most separations despite the presence of electroosmotic flow. The potential for application to very dilute sample solutions is demonstrated by detection of analytes having 10/sup /minus/9/ M concentrations, with signal to noise ratios of approximately 10/sup 2/ for some components, which as at least 2 orders of magnitude better than CZE/MS. CITP/MS appears to be an attractive complement to CZE/MS for dilute (low ionic strength) solutions since much greater sample sizes can be addressed without loss of efficiency.

• Research Organization: Pacific Northwest Lab., Richland, WA (USA)
• DOE Contract Number: AC06-76RL01830
• OSTI ID: 6190260
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 61:3; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102 -- Chemical & Spectral Procedures
400105* -- Separation Procedures
AMINES
AMINO ACIDS
AMMONIUM COMPOUNDS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BLOOD VESSELS
BODY
CAPILLARIES
CARBOXYLIC ACIDS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
CATIONS
CHARGED PARTICLES
CHEMICAL ANALYSIS
DATA
DISPERSIONS
DOPAMINE
DRUGS
EFFICIENCY
ELECTROPHORESIS
EQUIPMENT INTERFACES
EXPERIMENTAL DATA
HYDROXY COMPOUNDS
INFORMATION
IONS
ISOTACHOPHORESIS
MASS SPECTROSCOPY
MIXTURES
NEUROREGULATORS
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHENOLS
PHOSPHORUS COMPOUNDS
POLYPHENOLS
QUANTITATIVE CHEMICAL ANALYSIS
RESOLUTION
SALTS
SEPARATION PROCESSES
SOLUTIONS
SPECTROSCOPY
SYMPATHOMIMETICS
TRACE AMOUNTS