The on-line combination of capillary isotachophoresis with mass spectrometry

Conference · Mon May 01 04:00:00 EDT 1989

OSTI ID:5911646

Udseth, H. R.; Fields, S. M.; Loo, J. A.; Smith, R. D.

Capillary zone electrophoresis (CZE) has been demonstrated to have tremendous potential for high-resolution separations of ionic compounds of biological interest. The small capillary diameter (<100 ..mu..m) used in CZE and the need to keep the ionic strength of the sample two orders of magnitude less than that of the buffer place rather stringent limits on the amount of sample that can be injected. Isotachophoresis in a capillary format (CITP) is a complementary scheme that overcomes these limitations (1). The CITP/MS combination has several attractive features. First, there are no fundamental limits on the sample size that can be introduced. Second, CITP can result in the concentration of samples that are injected in dilute solutions. Third, once the separation is developed the sample elutes continuously, allowing efficient use of detector time. Fourth, the ideal peak shape in CITP is sharp sided and flat topped. This is an ideal peak shape for mass spectrometric detection. The scan speed of most mass spectrometers is not challenged and MS/MS analysis is facilitated (2,3). Electrospray ionization (ESI) has been shown to be an effective means of ionizing labile biopolymers from solution. In this work we present the results of an examination of two systems using CITP coupled to mass spectrometry by an ESI interface of our design (2).

• Research Organization: Pacific Northwest Lab., Richland, WA (USA)
• DOE Contract Number: AC06-76RL01830
• OSTI ID: 5911646
• Report Number(s): PNL-SA-16629; CONF-8905166-4; ON: DE89014275
• Country of Publication: United States
• Language: English

Related Subjects

440300* -- Miscellaneous Instruments-- (-1989)
47 OTHER INSTRUMENTATION
BIOLOGICAL MATERIALS
COMPARATIVE EVALUATIONS
ELECTROPHORESIS
INTERACTIONS
MASS SPECTROMETERS
MATERIALS
MEASURING INSTRUMENTS
SEPARATION PROCESSES
SPECTROMETERS